Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe
文献类型：期刊论文
| 作者 | Tian, Ruijun1,2,3; Li, Lingdong2,3; Tang, Wei2,3; Liu, Hongwei1,2,3; Ye, Mingliang1,2; Zhao, Zongbao K.2; Zou, Hanfa1,2 |
| 刊名 | proteomics
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 8期号:15页码:3094-3104 |
| 关键词 | 2-D nano LC chemical probe interactome isoprenoid chain MS/MS yeast proteome |
| ISSN号 | 1615-9853 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | a chemical proteomic approach was developed for profiling the noncovalent interactome of isoprenoid chain in the yeast proteome. a chemical probe that harbors a biotin moiety and a photoreactive benzophenone group linked to the terminal of geranyl group was synthesized. photoaffinity labeling was performed by incubating the saccharomyces cerevisiae proteome and the probe under 365 nm. uv light. thirty proteins were identified by immobilized neutraavidin enrichment, on-bead digestion, online 2-d nano-lc/ms/ms identification and semi-quantitative proteomic analysis. as noted by gene ontology annotation, the identified proteins demonstrate a wide range of catalytic activity in several biological processes, especially in metabolism and biosynthesis. further data analysis shows that hydrophobic binding of the synthetic probe is potentially the major interaction force leading to covalent labeling. these results argue that intracellular allosteric interactions conferred by the isoprenoid chain of the corresponding chemical structures may be widespread at an interactomic level. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | activated protein-kinase ; virus-rna replication ; fatty-acids ; saccharomyces-cerevisiae ; diphosphate analogs ; mevalonate kinase ; mass-spectrometry ; allosteric sites ; identification ; prenyltransferases |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000258503400011 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/100133]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Sch, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Ruijun,Li, Lingdong,Tang, Wei,et al. Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe[J]. proteomics,2008,8(15):3094-3104. |
| APA | Tian, Ruijun.,Li, Lingdong.,Tang, Wei.,Liu, Hongwei.,Ye, Mingliang.,...&Zou, Hanfa.(2008).Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe.proteomics,8(15),3094-3104. |
| MLA | Tian, Ruijun,et al."Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe".proteomics 8.15(2008):3094-3104. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。