The highly selective capture of phosphopeptides by zirconium phosphonate-modified magnetic nanoparticles for phosphoproteome analysis
文献类型：期刊论文
| 作者 | Zhao, Liang; Wu, Ren'an; Han, Guanghui; Zhou, Houjiang; Ren, Lianbing; Tian, Ruijun; Zou, Hanfa |
| 刊名 | journal of the american society for mass spectrometry
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 19期号:8页码:1176-1186 |
| ISSN号 | 1044-0305 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | the highly selective capture of phosphopeptides from proteolytic digests is a great challenge for the identification of phosphoproteins by mass spectrometry. in this work, the zirconium phosphonate-modified magnetic fe3o4/sio2 core/shell nanoparticles have been synthesized and successfully applied for the selective capture of phosphopeptides from complex tryptic digests of proteins before the analysis of maldi-tof mass spectrometry with the desired convenience of sample handling. the ratio of magnetic nanoparticle to protein and the incubation time for capturing phosphopeptides from complex proteolytic digests were investigated, and the optimized nanoparticle-to-protein ratio and incubation time were between 15:1 to 30:1 and 30 min, respectively. the excellent detection limit of 0.5 fmol beta-casein has been achieved by maldi-tof mass spectrometry with the specific capture of zirconium phosphonate-modified magnetic fe3o4 nanoparticles. the great specificity of zirconium phosphonate-modified magnetic fe3o4 nanoparticles to phosphopeptides was demonstrated by the selective capture of phosphopeptides from a complex tryptic digest of the mixture of a-casein and bovine serum albumin at molar ratio of 1 to 100 in maldi-tof-ms analysis. an application of the magnetic nanoparticles to selective capture phosphopeptides from a tryptic digest of mouse liver lysate was further carried out by combining with nano-lc-ms/ms and ms/ms/ms analyses, and a total of 194 unique phosphopeptides were successfully identified. |
| WOS标题词 | science & technology ; physical sciences ; technology |
| 类目[WOS] | chemistry, analytical ; chemistry, physical ; spectroscopy |
| 研究领域[WOS] | chemistry ; spectroscopy |
| 关键词[WOS] | protein identification technology ; fe(iii) affinity-chromatography ; large-scale analysis ; mass-spectrometry ; phosphorylated proteins ; proteomic analysis ; ms analysis ; enrichment ; peptides ; particles |
| 收录类别 | ISTP ; SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000258538500018 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/100153]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Liang,Wu, Ren'an,Han, Guanghui,et al. The highly selective capture of phosphopeptides by zirconium phosphonate-modified magnetic nanoparticles for phosphoproteome analysis[J]. journal of the american society for mass spectrometry,2008,19(8):1176-1186. |
| APA | Zhao, Liang.,Wu, Ren'an.,Han, Guanghui.,Zhou, Houjiang.,Ren, Lianbing.,...&Zou, Hanfa.(2008).The highly selective capture of phosphopeptides by zirconium phosphonate-modified magnetic nanoparticles for phosphoproteome analysis.journal of the american society for mass spectrometry,19(8),1176-1186. |
| MLA | Zhao, Liang,et al."The highly selective capture of phosphopeptides by zirconium phosphonate-modified magnetic nanoparticles for phosphoproteome analysis".journal of the american society for mass spectrometry 19.8(2008):1176-1186. |
入库方式： OAI收割
来源：大连化学物理研究所
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