Monitoring microbial populations of sulfate-reducing bacteria using an impedimetric immunosensor based on agglutination assay
文献类型：期刊论文
| 作者 | Wan, Yi1,2; Zhang, Dun1; Hou, Baorong1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2009-11-15 |
| 卷号 | 80期号:1页码:218-223 |
| 关键词 | Microbial Population Sulfate-reducing Bacteria Impedimetric Immunosensor Agglutination Assay Lectin |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2009.06.057 |
| 文献子类 | Article |
| 英文摘要 | An impedimetric immunosensor was fabricated for rapid and non-labeled detection of sulfate-reducing bacteria, Desulforibrio caledoiensis (SRB) by immobilizing lectin-Concanavalin A using an agglutination assay. The immobilization of lectin was conducted using amine coupling on the surface of a gold (Au) electrode assembled with 11-Mercaptounclecanoic acid. Electrochemical impedance spectroscopy (EIS) was used to verify the stepwise assembly of the sensor system. The work conditions of the impedimetric immunosensor, such as pH of the buffer solutions and the incubation time of lectin, were optimized. Faradic impedance spectra for charge transfer for the redox probe Fe(CN)(6)(3-/4-) were measured to determine SRB concentrations. The diameter of the Nyquist diagram that is equal to the charge-transfer resistance (RI) increased with increasing SRB concentration. A linear relationship between R(ct) and SRB concentration was obtained in SRB concentration range of 1.8 to 1.8 x 10(7) cfu/ml. The variation of the SRB population during the growth process was also monitored using the impedimetric immunosensor. This approach has great potential for simple, low-cost. and time-saving monitoring of microbial populations. (C) 2009 Elsevier B.V. All rights reserved.; An impedimetric immunosensor was fabricated for rapid and non-labeled detection of sulfate-reducing bacteria, Desulforibrio caledoiensis (SRB) by immobilizing lectin-Concanavalin A using an agglutination assay. The immobilization of lectin was conducted using amine coupling on the surface of a gold (Au) electrode assembled with 11-Mercaptounclecanoic acid. Electrochemical impedance spectroscopy (EIS) was used to verify the stepwise assembly of the sensor system. The work conditions of the impedimetric immunosensor, such as pH of the buffer solutions and the incubation time of lectin, were optimized. Faradic impedance spectra for charge transfer for the redox probe Fe(CN)(6)(3-/4-) were measured to determine SRB concentrations. The diameter of the Nyquist diagram that is equal to the charge-transfer resistance (RI) increased with increasing SRB concentration. A linear relationship between R-ct and SRB concentration was obtained in SRB concentration range of 1.8 to 1.8 x 10(7) cfu/ml. The variation of the SRB population during the growth process was also monitored using the impedimetric immunosensor. This approach has great potential for simple, low-cost. and time-saving monitoring of microbial populations. (C) 2009 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry, Analytical |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000271055700033 |
| 公开日期 | 2010-12-21 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/2273]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Yi,Zhang, Dun,Hou, Baorong. Monitoring microbial populations of sulfate-reducing bacteria using an impedimetric immunosensor based on agglutination assay[J]. TALANTA,2009,80(1):218-223. |
| APA | Wan, Yi,Zhang, Dun,&Hou, Baorong.(2009).Monitoring microbial populations of sulfate-reducing bacteria using an impedimetric immunosensor based on agglutination assay.TALANTA,80(1),218-223. |
| MLA | Wan, Yi,et al."Monitoring microbial populations of sulfate-reducing bacteria using an impedimetric immunosensor based on agglutination assay".TALANTA 80.1(2009):218-223. |
入库方式： OAI收割
来源：海洋研究所
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