Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter
文献类型：期刊论文
| 作者 | Wang, Fangjun; Ye, Mingliang; Dong, Jing; Tian, Ruijun; Hu, Lianghai; Han, Guanghui; Jiang, Xinning; Wu, Ren'an; Zou, Hanfa |
| 刊名 | journal of separation science
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 31期号:14页码:2589-2597 |
| 关键词 | hydrophobic monolith label-free quantitative proteome analysis monolithic ESI emitter mouse liver nano-UPLC-MS/MSE |
| ISSN号 | 1615-9306 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | the postcolumn void volume, which is introduced by the connecting tubing and void esi emitter in the nanoflow lc coupled with ms/ms system (mu lc-ms/ms), is harmful for the analysis of peptides in shotgun proteome analysis. a new type of porous c12 monolithic esi emitter was prepared to eliminate the disruption and mixing effects occurring in the connecting tubing and void emitter. it was demonstrated that the porous hydrophobic monolith inside the emitter played a key role in retaining the good peak profile, and the average peak capacity of the whole separation system increased 12.8% in contrast to commercially available void emitter. then, the porous cl 2 monolithic emitter was applied in label-free quantitative proteome analysis of two standard protein mixtures that were spiked into the tryptic digest of mouse livers extract. compared to commercially available void esi emitter, the number of proteins with reliable results in quantification increased greatly. and the relative quantities of the four standard proteins were all determined with the relative error <= 6.8%. however, quantitative information of only three standard proteins could be obtained when void emitter was used. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | spectrometry-based proteomics ; organic polymer monoliths ; tandem mass-spectrometry ; coded affinity tags ; ion-trap ; biomarker discovery ; shotgun proteomics ; stationary phases ; quantification ; proteins |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000258668000008 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/100175]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fangjun,Ye, Mingliang,Dong, Jing,et al. Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter[J]. journal of separation science,2008,31(14):2589-2597. |
| APA | Wang, Fangjun.,Ye, Mingliang.,Dong, Jing.,Tian, Ruijun.,Hu, Lianghai.,...&Zou, Hanfa.(2008).Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter.journal of separation science,31(14),2589-2597. |
| MLA | Wang, Fangjun,et al."Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter".journal of separation science 31.14(2008):2589-2597. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。