Selective and specific detection of sulfate-reducing bacteria using potentiometric stripping analysis
文献类型：期刊论文
| 作者 | Wan, Yi2; Zhang, Dun1; Hou, Baorong |
| 刊名 | TALANTA
 |
| 出版日期 | 2010-09-15 |
| 卷号 | 82期号:4页码:1608-1611 |
| ISSN号 | 0039-9140 |
| 关键词 | Potentiometric Stripping Analysis Sulfate-reducing Bacteria Pathogenic Bacteria Detection Sulfide |
| DOI | 10.1016/j.talanta.2010.07.030 |
| 文献子类 | Article |
| 英文摘要 | A fast, sensitive and reliable potentiometric stripping analysis (PSA) is described for the selective detection of the marine pathogenic sulfate-reducing bacterium (SRB). Desulforibrio caledoiensis. The chemical and electrochemical parameters that exert influence on the deposition and stripping of lead ion, such as deposition potential, deposition time and pH value were carefully studied. The concentration of SRB was determined in acetate buffer solution (pH 5.2) under the optimized condition (deposition potential of -1.3 V. deposition time of 250 s, ionic strength of 0.2 mol L(-1) and oxidant mercury (II) concentration of 40 mg L(-1)). A linear relationship between the stripping response and the logarithm of the bacterial concentration was observed in the range of 2.3 x 10 to 2.3 x 10(7) cfu mL(-1). In addition, the potentiometric stripping technique gave a distinct response to the SRB, but had no obvious response to Escherichia coli. The measurement system has a potential for further applications and provides a facile and sample method for detection of pathogenic bacteria. (C) 2010 Elsevier B.V. All rights reserved.; A fast, sensitive and reliable potentiometric stripping analysis (PSA) is described for the selective detection of the marine pathogenic sulfate-reducing bacterium (SRB). Desulforibrio caledoiensis. The chemical and electrochemical parameters that exert influence on the deposition and stripping of lead ion, such as deposition potential, deposition time and pH value were carefully studied. The concentration of SRB was determined in acetate buffer solution (pH 5.2) under the optimized condition (deposition potential of -1.3 V. deposition time of 250 s, ionic strength of 0.2 mol L-1 and oxidant mercury (II) concentration of 40 mg L-1). A linear relationship between the stripping response and the logarithm of the bacterial concentration was observed in the range of 2.3 x 10 to 2.3 x 10(7) cfu mL(-1). In addition, the potentiometric stripping technique gave a distinct response to the SRB, but had no obvious response to Escherichia coli. The measurement system has a potential for further applications and provides a facile and sample method for detection of pathogenic bacteria. (C) 2010 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry, Analytical |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000282480500080 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5193]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Yi,Zhang, Dun,Hou, Baorong. Selective and specific detection of sulfate-reducing bacteria using potentiometric stripping analysis[J]. TALANTA,2010,82(4):1608-1611. |
| APA | Wan, Yi,Zhang, Dun,&Hou, Baorong.(2010).Selective and specific detection of sulfate-reducing bacteria using potentiometric stripping analysis.TALANTA,82(4),1608-1611. |
| MLA | Wan, Yi,et al."Selective and specific detection of sulfate-reducing bacteria using potentiometric stripping analysis".TALANTA 82.4(2010):1608-1611. |
入库方式： OAI收割
来源：海洋研究所
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