Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis
文献类型：期刊论文
| 作者 | Zhou, Houjiang; Ye, Mingliang; Dong, Jing; Han, Guanghui; Jiang, Xinning; Wu, Renan; Zou, Hanfa |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2008-09-01 |
| 卷号 | 7期号:9页码:3957-3967 |
| 关键词 | immobilized metal ion affinity chromatography titanium ion phosphate polymer phosphopeptide enrichment phosphoproteome analysis |
| ISSN号 | 1535-3893 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | the elucidation of protein post-translational modifications, such as phosphorylation, remains a challenging analytical task for proteomic studies. since many of the proteins targeted for phosphorylation are low in abundance and phosphorylation is typically substoichiometric, a prerequisite for their identification is the specific enrichment of phosphopeptide prior to mass spectrometric analysis. here, we presented a new method termed as immobilized titanium ion affinity chromatography (ti4+-imac) for enriching phosphopeptides. a phosphate polymer, which was prepared by direct polymerization of monomers containing phosphate groups, was applied to immobilize ti4+ through the chelating interaction between phosphate groups on the polymer and ti4+. the resulting ti4+-imac resin specifically isolates phosphopeptides from a digest mixture of standard phosphoproteins and nonphosphoprotein (bsa) in a ratio as low as 1:500. ti4+-imac was further applied for phosphoproteome analysis of mouse liver. we also compared ti4+-imac to other enrichment methods including fe3+-imac, zr4+-imac, tio2 and zrcl2 and demonstrate superior selectivity and efficiency of ti4+-imac for the isolation and enrichment of phosphopeptides. the high specificity and efficiency of phosphopeptide enrichment by ti4+-imac mainly resulted from the flexibility of immobilized titanium ion with spacer arm linked to polymer beads as well as the specific interaction between immobilized titanium ion and phosphate group on phosphopeptides. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | tandem mass-spectrometry ; scale phosphorylation analysis ; highly selective enrichment ; nano-lc-ms/ms ; saccharomyces-cerevisiae ; magnetic nanoparticles ; methyl esterification ; peptide mixtures ; mouse-liver ; ms analysis |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000259015500031 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/100199]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Houjiang,Ye, Mingliang,Dong, Jing,et al. Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis[J]. journal of proteome research,2008,7(9):3957-3967. |
| APA | Zhou, Houjiang.,Ye, Mingliang.,Dong, Jing.,Han, Guanghui.,Jiang, Xinning.,...&Zou, Hanfa.(2008).Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis.journal of proteome research,7(9),3957-3967. |
| MLA | Zhou, Houjiang,et al."Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis".journal of proteome research 7.9(2008):3957-3967. |
入库方式： OAI收割
来源：大连化学物理研究所
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