Multiplexed profiling of single-cell extracellular vesicles secretion
文献类型：期刊论文
| 作者 | Ji, Yahui1,2; Qi, Dongyuan3; Li, Linmei1; Su, Haoran1,2; Li, Xiaojie2; Luo, Yong4; Sun, Bo5; Zhang, Fuyin5; Lin, Bingcheng1; Liu, Tingjiao2 |
| 刊名 | Proceedings of the national academy of sciences of the united states of america
 |
| 出版日期 | 2019-03-26 |
| 卷号 | 116期号:13页码:5979-5984 |
| ISSN号 | 0027-8424 |
| 关键词 | Single-cell analysis Extracellular vesicle Cellular heterogeneity Antibody barcodes |
| DOI | 10.1073/pnas.1814348116 |
| 通讯作者 | Liu, tingjiao(tingjiao@dmu.edu.cn) ; Lu, yao(luyao@dicp.ac.cn) |
| 英文摘要 | Extracellular vesicles (evs) are important intercellular mediators regulating health and diseases. conventional methods for ev surface marker profiling, which was based on population measurements, masked the cell-to-cell heterogeneity in the quantity and phenotypes of ev secretion. herein, by using spatially patterned antibody barcodes, we realized multiplexed profiling of single-cell ev secretion from more than 1,000 single cells simultaneously. applying this platform to profile human oral squamous cell carcinoma (oscc) cell lines led to a deep understanding of previously undifferentiated single-cell heterogeneity underlying ev secretion. notably, we observed that the decrement of certain ev phenotypes (e.g., (cd63+) v) was associated with the invasive feature of both oscc cell lines and primary oscc cells. we also realized multiplexed detection of ev secretion and cytokines secretion simultaneously from the same single cells to investigate the multidimensional spectrum of cellular communications, from which we resolved tiered functional subgroups with distinct secretion profiles by visualized clustering and principal component analysis. in particular, we found that different cell subgroups dominated ev secretion and cytokine secretion. the technology introduced here enables a comprehensive evaluation of ev secretion heterogeneity at single-cell level, which may become an indispensable tool to complement current single-cell analysis and ev research. |
| WOS关键词 | CD63 TETRASPANIN ; EXOSOME SECRETION ; HETEROGENEITY ; REVEALS ; EXPRESSION ; MICROVESICLES ; CARCINOMA ; CYTOKINE ; RELEASE |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| 出版者 | NATL ACAD SCIENCES |
| WOS记录号 | WOS:000462382800029 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2372626 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Liu, Tingjiao; Lu, Yao |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 2.Dalian Med Univ, Coll Stomatol, Dalian 116044, Peoples R China 3.Dalian Med Univ, Affiliated Hosp 1, Dalian 116011, Peoples R China 4.Dalian Univ Technol, Dept Chem Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China 5.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Yahui,Qi, Dongyuan,Li, Linmei,et al. Multiplexed profiling of single-cell extracellular vesicles secretion[J]. Proceedings of the national academy of sciences of the united states of america,2019,116(13):5979-5984. |
| APA | Ji, Yahui.,Qi, Dongyuan.,Li, Linmei.,Su, Haoran.,Li, Xiaojie.,...&Lu, Yao.(2019).Multiplexed profiling of single-cell extracellular vesicles secretion.Proceedings of the national academy of sciences of the united states of america,116(13),5979-5984. |
| MLA | Ji, Yahui,et al."Multiplexed profiling of single-cell extracellular vesicles secretion".Proceedings of the national academy of sciences of the united states of america 116.13(2019):5979-5984. |
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来源：大连化学物理研究所
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