A multiplex fragment-ion-based method for accurate proteome quantification
文献类型：期刊论文
| 作者 | Liu, Jianhui1,2; Zhou, Yuan1,3; Shan, Yichu1; Zhao, Baofeng1; Hu, Yechen1,2; Sui, Zhigang1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2019-03-19 |
| 卷号 | 91期号:6页码:3921-3928 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b04806 |
| 通讯作者 | Zhang, lihua(lihuazhang@dicp.ac.cn) |
| 英文摘要 | Multiplex proteome quantification with high accuracy is urgently required to achieve a comprehensive understanding of dynamic cellular and physiological processes. among the existing quantification strategies, fragment-ion based methods can provide highly accurate results, but the multiplex capacity is limited to 3-plex. herein, we developed a multiplex pseudo-isobaric dimethyl labeling (m-pidl) method to extend the capacity of the fragment-ion-based method to 6-plex by one-step dimethyl labeling with several millidalton and dalton mass differences between precursor ions and enlarging the isolation window of precursor ions to 10 m/z during data acquisition. m-pidl showed high quantification accuracy within the 20-fold dynamic range. notably, the ratio compression was 1.13-fold in a benchmark two-proteome model (5:1 mixed e. coli proteins with hela proteins as interference), indicating that by m-pidl, the ratio distortion of isobaric labeling approaches and the approximate 40% ratio shift of the label free quantification strategy could be effectively eliminated. additionally, m-pidl did not show ratio variation among post translational modifications (cv = 6.66%), which could benefit the measurement of universal protein properties for proteomic atlases. we further employed m-pidl to monitor the time-resolved responses of the tgf-beta-induced epithelial-mesenchymal transition (emt) in lung adenocarcinoma a549 cell lines, which facilitated the finding of new potential regulatory proteins. therefore, the 6-plex quantification of m-pidl with the remarkably high accuracy might create new prospects for comprehensive proteome analysis. |
| WOS关键词 | TANDEM MASS TAGS ; COMPUTATIONAL PLATFORM ; CANCER ; SPECTROMETRY ; EXPRESSION ; PROTEINS ; STRATEGY ; EMT |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000462098300023 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2372628 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Zhang, Lihua |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Xuzhou Med Univ, Sch Med Technol, Xuzhou 221004, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jianhui,Zhou, Yuan,Shan, Yichu,et al. A multiplex fragment-ion-based method for accurate proteome quantification[J]. Analytical chemistry,2019,91(6):3921-3928. |
| APA | Liu, Jianhui.,Zhou, Yuan.,Shan, Yichu.,Zhao, Baofeng.,Hu, Yechen.,...&Zhang, Yukui.(2019).A multiplex fragment-ion-based method for accurate proteome quantification.Analytical chemistry,91(6),3921-3928. |
| MLA | Liu, Jianhui,et al."A multiplex fragment-ion-based method for accurate proteome quantification".Analytical chemistry 91.6(2019):3921-3928. |
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来源：大连化学物理研究所
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