A new searching strategy for the identification of o-linked glycopeptides
文献类型：期刊论文
| 作者 | Mao, Jiawei1,2; You, Xin1,2; Qin, Hongqiang1,2; Wang, Chenye3; Wang, Liming3; Ye, Mingliang1,2 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2019-03-19 |
| 卷号 | 91期号:6页码:3852-3859 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b04184 |
| 通讯作者 | Ye, mingliang(mingliang@dicp.ac.cn) |
| 英文摘要 | For the analysis of homogeneous post-translational modifications such as protein phosphorylation and acetylation, setting a variable modification on the specific residue(s) is applied to identify the modified peptides for database searching. however, this approach is often not applicable to identify intact mucin-type o-glycopeptides due to the high microheterogeneity of the glycosylation. because there is virtually no carbohydrate-related tag on the peptide fragments after the o-glycopeptides are dissociated in hcd, we find it is unnecessary to set the variable mass tags on the ser/thr residues to identify the peptide sequences. in this study, we present a novel approach, termed as o-search, for the interpretation of o-glycopeptide hcd spectra. instead of setting the variable mass tags on the ser/thr residues, we set variable mass tags on the peptide level. the precursor mass of the ms/ms spectrum was deducted by every possible summed mass of o-glycan combinations on at most three s/t residues. all the spectra with these new precursor masses were searched against the protein sequence database without setting variable glycan modifications. it was found that this method had much decreased search space and had excellent sensitivity in the identification of o-glycopeptides. compared with the conventional searching approach, o-search yielded 96%, 86%, and 79% improvement in glycopeptide spectra matching, glycopeptide identification, and peptide sequence identification, respectively. it was demonstrated that o-search enabled the consideration of more glycan structures and was fitted to analyze microheterogeneity of o-glycosylation. |
| WOS关键词 | INTACT N-GLYCOPEPTIDES ; GLYCOSYLATION ; GLYCOPROTEOME ; PROTEOMICS ; SPECTRA ; GLYCANS ; ROLES |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000462098300015 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2372640 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Ye, Mingliang |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Med Univ, Hosp 2, Dept Surg, Div Hepatobiliary & Pancreat Surg, Dalian 116023, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mao, Jiawei,You, Xin,Qin, Hongqiang,et al. A new searching strategy for the identification of o-linked glycopeptides[J]. Analytical chemistry,2019,91(6):3852-3859. |
| APA | Mao, Jiawei,You, Xin,Qin, Hongqiang,Wang, Chenye,Wang, Liming,&Ye, Mingliang.(2019).A new searching strategy for the identification of o-linked glycopeptides.Analytical chemistry,91(6),3852-3859. |
| MLA | Mao, Jiawei,et al."A new searching strategy for the identification of o-linked glycopeptides".Analytical chemistry 91.6(2019):3852-3859. |
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来源：大连化学物理研究所
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