Engineering human islet organoids from ipscs using an organ-on-chip platform
文献类型：期刊论文
| 作者 | Tao, Tingting1,4; Wang, Yaqing1,4; Chen, Wenwen1,4; Li, Zhongyu1; Su, Wentao1; Guo, Yaqiong1,4; Deng, Pengwei1,4; Qin, Jianhua1,2,3,4 |
| 刊名 | Lab on a chip
 |
| 出版日期 | 2019-03-21 |
| 卷号 | 19期号:6页码:948-958 |
| ISSN号 | 1473-0197 |
| DOI | 10.1039/c8lc01298a |
| 通讯作者 | Qin, jianhua(jhqin@bdicp.ac.cn) |
| 英文摘要 | Human pluripotent stem cell (hpsc)-derived islet cells provide promising resources for diabetes studies, cell replacement treatment and drug screening. recently, hpsc-derived organoids have represented a new class of in vitro organ models for disease modeling and regenerative medicine. however, rebuilding biomimetic human islet organoids from hpscs remains challenging. here, we present a new strategy to engineer human islet organoids derived from human induced pluripotent stem cells (hipscs) using an organ-on-a-chip platform combined with stem cell developmental principles. the microsystem contains a multi-layer microfluidic device that allows controllable aggregation of embryoid bodies (ebs), in situ pancreatic differentiation and generation of heterogeneous islet organoids in parallel under perfused 3d culture in a single device. the generated islet organoids contain heterogeneous islet-specific a and beta-like cells that exhibit favorable growth and cell viability. they also show enhanced expression of pancreatic beta-cell specific genes and proteins (pdx1 and nkx6.1) and increased beta-cell hormone specific ins gene and c-peptide protein expressions under perfused 3d culture conditions compared to static cultures. in addition, the islet organoids exhibit more sensitive glucose-stimulated insulin secretion (gsis) and higher ca2+ flux, indicating the role of biomimetic mechanical flow in promoting endocrine cell differentiation and maturation of islet organoids. this islet-on-a-chip system is robust and amenable to real-time imaging and in situ tracking of islet organoid growth, which may provide a promising platform for organoid engineering, disease modeling, drug testing and regenerative medicine. |
| WOS关键词 | PANCREATIC BETA-CELLS ; STEM-CELLS ; A-CHIP ; GENERATION ; MODEL ; DIFFERENTIATION ; ORGANOGENESIS ; MATURATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics |
| WOS类目 | Biochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| 出版者 | ROYAL SOC CHEMISTRY |
| WOS记录号 | WOS:000462666200017 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2372649 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Qin, Jianhua |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China 3.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tao, Tingting,Wang, Yaqing,Chen, Wenwen,et al. Engineering human islet organoids from ipscs using an organ-on-chip platform[J]. Lab on a chip,2019,19(6):948-958. |
| APA | Tao, Tingting.,Wang, Yaqing.,Chen, Wenwen.,Li, Zhongyu.,Su, Wentao.,...&Qin, Jianhua.(2019).Engineering human islet organoids from ipscs using an organ-on-chip platform.Lab on a chip,19(6),948-958. |
| MLA | Tao, Tingting,et al."Engineering human islet organoids from ipscs using an organ-on-chip platform".Lab on a chip 19.6(2019):948-958. |
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来源：大连化学物理研究所
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