Simultaneous quantification of ten oxysterols based on lc-ms/ms and its application in atherosclerosis human serum samples
文献类型：期刊论文
| 作者 | Chen, Lishan1; Xiu, Rui2; Wang, Huan1; Wang, Longxing3; Wu, Guanmin1; Liang, Jian1; Han, Xiaofei1 |
| 刊名 | Chromatographia
 |
| 出版日期 | 2019-02-01 |
| 卷号 | 82期号:2页码:553-564 |
| 关键词 | Lc-ms Ms Oxysterols Human serum Derivatization Atherosclerosis |
| ISSN号 | 0009-5893 |
| DOI | 10.1007/s10337-018-3654-6 |
| 通讯作者 | Han, xiaofei(helarpmi1640@126.com) |
| 英文摘要 | A highly sensitive and specific liquid chromatography-tandem mass spectrometric (lc-ms/ms) method has been developed and validated for the identification and relative quantification of ten oxysterols in human serum. after alkaline hydrolysis and derivatization, oxysterols in 50 mu l human serum were extracted and converted to corresponding picolinyl ester derivatives. the freshly prepared samples were analyzed by lc-ms/ms in high efficiency; and these ten oxysterols were separated within 11min. the method enabled the determination of standards with limits of detection between 0.0048 and 0.14ng/ml. the variances between sample preparations and measurements by this method were 3.28-8.47% and 2.01-7.26%, respectively. recoveries of the oxysterols and cholesterol ranged from 90.49 to 121.01%, with the mean recovery of 102.63%. the rapid lc-ms/ms method enables relative quantification of oxysterols in human serum under high-throughput conditions. furthermore, the established method has been applied to analyze the content of ten oxysterols in serum of healthy people and atherosclerotic. the results showed that the levels of 25-hydroxycholesterol and cholestane-3 ss,5,6 ss-triol in patients were significantly different from healthy people. this current method might provide the opportunity for clinical application. [graphics] . |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; LIVER X RECEPTOR ; LIPOPROTEIN ; OXIDATION ; PRODUCTS ; STEROLS ; PLASMA ; DERIVATIVES ; EXTRACTION ; DISEASE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000460411700007 |
| 出版者 | SPRINGER HEIDELBERG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2372664 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Han, Xiaofei |
| 作者单位 | 1.Dalian Univ, Key Lab Carbohydrate & Lipid Metab Res, Coll Life Sci & Technol, 10 Xuefu Ave, Dalian 116622, Liaoning, Peoples R China 2.Dalian Sanat, Dept Pharm, Dalian 116013, Liaoning, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Lishan,Xiu, Rui,Wang, Huan,et al. Simultaneous quantification of ten oxysterols based on lc-ms/ms and its application in atherosclerosis human serum samples[J]. Chromatographia,2019,82(2):553-564. |
| APA | Chen, Lishan.,Xiu, Rui.,Wang, Huan.,Wang, Longxing.,Wu, Guanmin.,...&Han, Xiaofei.(2019).Simultaneous quantification of ten oxysterols based on lc-ms/ms and its application in atherosclerosis human serum samples.Chromatographia,82(2),553-564. |
| MLA | Chen, Lishan,et al."Simultaneous quantification of ten oxysterols based on lc-ms/ms and its application in atherosclerosis human serum samples".Chromatographia 82.2(2019):553-564. |
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来源：大连化学物理研究所
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