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Various multicharged anions of ginsenosides in negative electrospray ionization with qtof high-resolution mass spectrometry

文献类型：期刊论文

作者Zhao, Nan1,2; Cheng, Mengchun1; Huang, Shuai1; Liu, Dan1; Zhao, Qiang1,3; Bai, Yunpeng1; Zhang, Xiaozhe1
刊名Journal of the american society for mass spectrometry
出版日期2019-03-01
卷号30期号:3页码:403-418
ISSN号1044-0305
关键词Electrospray ionization Ginsenoside Multicharged anion Multimer High-resolution mass spectrometry
DOI10.1007/s13361-018-2089-5
通讯作者Zhang, xiaozhe(zhangxz@dicp.ac.cn)
英文摘要When characterizing components from ginseng, we found a vast number of multicharged anions presented in the liquid chromatography-mass spectrometry (lc-ms) chromatograms. the source of these anions is unclear yet, while ginsenosides, the major components of ginseng, are the main suspected type of molecules because of their sugar moiety. our investigation using 14 pure ginsenosides affirmed that the multicharged anions were formed by ginsenosides rather than other types of ingredients in ginseng. various anions could be observed for each ginsenoside. these anions contain ions ([m-2h](2-), [m+adduct](2-)), as well as those formed by polymerization of at least two ginsenosides, such as [nm-2h](2-), [nm-h+adduct](2-), and [nm-3h](3-). the presence of so different types of ions from a ginsenoside explains the reason for the large number of anions in the lc-ms analysis of ginseng. we further found that formation of [nm-2h](2-) ions was influenced by the number of sugar chains: ginsenosides containing two sugar chains produced all [nm-2h](2-) ion types, whereas ginsenosides containing one sugar chain did not produce [2m-2h](2-). thus, [2m-2h](2-) and [3m-2h](2-) can be utilized to rapidly identify monodesmosidic and/or bidesmosidic ginsenosides as joint diagnostic anions. the position of the glycosyl radical might be the key factor affecting the formation of multicharged multimer ions from monodesmosidic ginsenosides. consequently, three groups of ginsenoside isomers were differentiated by characteristic [nm-2h](2-) anions. using concentration-dependent characteristics and collision-induced dissociation (cid), we confirmed that [nm-2h](2-) ions are non-covalently bound multimers whose aggregation has marked distinction between monodesmosidic and bidesmosidic ginsenosides, accounting for the differentiated formation of [nm-2h](2-) between them.
WOS关键词PANAX-QUINQUEFOLIUS ; AMERICAN GINSENG ; ASIAN GINSENG ; DIFFERENTIATION ; DISCRIMINATION ; LEAVES ; RH2 ; RG1
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
WOS类目Biochemical Research Methods ; Chemistry, Analytical ; Chemistry, Physical ; Spectroscopy
语种英语
出版者SPRINGER
WOS记录号WOS:000461325300002
URI标识http://www.irgrid.ac.cn/handle/1471x/2372690
专题大连化学物理研究所
通讯作者Zhang, Xiaozhe
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Zhongshan Rd 457, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Yuquan Rd 19, Beijing 100049, Peoples R China
3.Shenyang Pharmaceut Univ, Minist Educ, Key Lab Struct Based Drug Design & Discovery, Shenyang, Liaoning, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Nan,Cheng, Mengchun,Huang, Shuai,et al. Various multicharged anions of ginsenosides in negative electrospray ionization with qtof high-resolution mass spectrometry[J]. Journal of the american society for mass spectrometry,2019,30(3):403-418.
APA Zhao, Nan.,Cheng, Mengchun.,Huang, Shuai.,Liu, Dan.,Zhao, Qiang.,...&Zhang, Xiaozhe.(2019).Various multicharged anions of ginsenosides in negative electrospray ionization with qtof high-resolution mass spectrometry.Journal of the american society for mass spectrometry,30(3),403-418.
MLA Zhao, Nan,et al."Various multicharged anions of ginsenosides in negative electrospray ionization with qtof high-resolution mass spectrometry".Journal of the american society for mass spectrometry 30.3(2019):403-418.

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