Combinatorial peptide ligand library-based photoaffinity probe for the identification of phosphotyrosine-binding domain proteins
文献类型：期刊论文
| 作者 | An, Jinying1; Zhai, Guijin1; Guo, Zhenchang1; Bai, Xue1; Chen, Pu1; Dong, Hanyang1; Tian, Shanshan1; Ai, Ding2; Zhang, Yukui3; Zhang, Kai1 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2019-03-05 |
| 卷号 | 91期号:5页码:3221-3226 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b04781 |
| 通讯作者 | Zhai, guijin(zhaiguijin@tmu.edu.cn) ; Zhang, kai(kzhang@tmu.edu.cn) |
| 英文摘要 | Phosphotyrosine (py) serves as a docking site for the recognition proteins containing py-binding (pyb) modules, such as the sh2 domain, to mediate cell signal transduction. thus, it is vital to profile these binding proteins for understanding of signal regulation. however, identification of pyb proteins remains a significant challenge due to their low abundance and typically weak and transient interactions with py sites. herein, we designed and prepared a py-peptide photoaffinity probe for the robust and specific enrichment and identification of its binding proteins. using shc1-py(317) as a paradigm, we showed that the developed probe enables to capture target protein with high selectivity and remarkable specificity even in a complex context. notably, we expanded the strategy to a combinatorial py-peptide-based photoaffinity probe by using combinatorial peptide ligand library (cpll) technique and identified 24 sh2 domain proteins, which presents a deeper profiling of pyb proteins than previous reports using affinity probes. moreover, the method can be used to mine putative pyb proteins and confirmed pkn2 as a selective binder to py, expanding the repertoire of known domain proteins. our approach provides a general strategy for rapid and robust interrogating pyb proteins and will promote the understanding of the signal transduction mechanism. |
| WOS关键词 | TYROSINE PHOSPHORYLATION ; SH2 DOMAIN ; SPECIFICITY ; PROTEOME ; TARGET ; SITES |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000460709200009 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2372720 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Zhai, Guijin; Zhang, Kai |
| 作者单位 | 1.Tianjin Med Univ, Canc Inst & Hosp,Collaborat Innovat Ctr Tianjin M, Tianjin Key Lab Med Epigenet,Key Lab Breast Canc, Key Lab Immune Microenvironm & Dis,Minist Educ,De, Tianjin 300070, Peoples R China 2.Tianjin Med Univ, Dept Physiol & Pathophysiol, Tianjin Key Lab Metab Dis, Tianjin 300070, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Jinying,Zhai, Guijin,Guo, Zhenchang,et al. Combinatorial peptide ligand library-based photoaffinity probe for the identification of phosphotyrosine-binding domain proteins[J]. Analytical chemistry,2019,91(5):3221-3226. |
| APA | An, Jinying.,Zhai, Guijin.,Guo, Zhenchang.,Bai, Xue.,Chen, Pu.,...&Zhang, Kai.(2019).Combinatorial peptide ligand library-based photoaffinity probe for the identification of phosphotyrosine-binding domain proteins.Analytical chemistry,91(5),3221-3226. |
| MLA | An, Jinying,et al."Combinatorial peptide ligand library-based photoaffinity probe for the identification of phosphotyrosine-binding domain proteins".Analytical chemistry 91.5(2019):3221-3226. |
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来源：大连化学物理研究所
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