中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of his-tagged protein

文献类型:期刊论文

作者Chang, Mengmeng1,2; Qin, Qian1,2; Wang, Bohong1,2; Xia, Tian1; Lv, Wangjie1,2; Sun, Xiaoshan1,2; Shi, Xianzhe1; Xu, Guowang1
刊名Journal of separation science
出版日期2019-02-01
卷号42期号:3页码:744-753
关键词Immobilized metal-ion affinity chromatography Layer-by-layer assembly Magnetic nanoparticles Protein purification
ISSN号1615-9306
DOI10.1002/jssc.201800969
通讯作者Shi, xianzhe(shixianzhe@dicp.ac.cn)
英文摘要Employing immobilized metal-ion affinity chromatography and magnetic separation could ideally provide a useful analytical strategy for purifying his-tagged protein. in the current study, a facile route was designed to prepare cmpei-ni2+@sio2@fe3o4 (cmpei=carboxymethylated polyethyleneimine) magnetic nanoparticles composed of a strong magnetic core of fe3o4 and a ni2+-immobilized carboxymethylated polyethyleneimine coated outside shell, which was formed by electrostatic interactions between polyanionic electrolyte of carboxymethylated polyethyleneimine and positively charged surface of 3-(trimethoxysilyl) propylamin modified sio2@fe3o4. the resulting cmpei-ni2+@sio2@fe3o4 composite nanoparticles displayed well-uniform structure and high magnetic responsiveness. hexa his-tagged peptides and purified his-tagged recombinant retinoid x receptor alpha were chosen as the model samples to evaluate the adsorption, capacity, and reusability of the composite nanoparticles. the results demonstrated the cmpei-ni2+@sio2@fe3o4 nanoparticles possessed rapid adsorption, large capacity, and good recyclability. the obtained nanoparticles were further used to purify his-tagged protein in practical environment. it was found that the nanoparticles could selectively capture his-tagged recombinant retinoid x receptor protein from complex cell lysate. owing to its easy synthesis, large binding capacity, and good reusability, the prepared cmpei-ni2+@sio2@fe3o4 magnetic nanoparticles have great potential for application in biotechnological fields.
WOS关键词SELECTIVE REMOVAL ; SEPARATION ; ADSORPTION ; EXPRESSION ; IMMOBILIZATION ; MICROSPHERES ; EXTRACTION ; COMPOSITE ; SILICA
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
语种英语
WOS记录号WOS:000457651000013
出版者WILEY-V C H VERLAG GMBH
URI标识http://www.irgrid.ac.cn/handle/1471x/2372814
专题大连化学物理研究所
通讯作者Shi, Xianzhe
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Chang, Mengmeng,Qin, Qian,Wang, Bohong,et al. Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of his-tagged protein[J]. Journal of separation science,2019,42(3):744-753.
APA Chang, Mengmeng.,Qin, Qian.,Wang, Bohong.,Xia, Tian.,Lv, Wangjie.,...&Xu, Guowang.(2019).Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of his-tagged protein.Journal of separation science,42(3),744-753.
MLA Chang, Mengmeng,et al."Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of his-tagged protein".Journal of separation science 42.3(2019):744-753.

入库方式: iSwitch采集

来源:大连化学物理研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。