Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of his-tagged protein
文献类型:期刊论文
作者 | Chang, Mengmeng1,2; Qin, Qian1,2; Wang, Bohong1,2; Xia, Tian1; Lv, Wangjie1,2; Sun, Xiaoshan1,2; Shi, Xianzhe1; Xu, Guowang1 |
刊名 | Journal of separation science
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出版日期 | 2019-02-01 |
卷号 | 42期号:3页码:744-753 |
关键词 | Immobilized metal-ion affinity chromatography Layer-by-layer assembly Magnetic nanoparticles Protein purification |
ISSN号 | 1615-9306 |
DOI | 10.1002/jssc.201800969 |
通讯作者 | Shi, xianzhe(shixianzhe@dicp.ac.cn) |
英文摘要 | Employing immobilized metal-ion affinity chromatography and magnetic separation could ideally provide a useful analytical strategy for purifying his-tagged protein. in the current study, a facile route was designed to prepare cmpei-ni2+@sio2@fe3o4 (cmpei=carboxymethylated polyethyleneimine) magnetic nanoparticles composed of a strong magnetic core of fe3o4 and a ni2+-immobilized carboxymethylated polyethyleneimine coated outside shell, which was formed by electrostatic interactions between polyanionic electrolyte of carboxymethylated polyethyleneimine and positively charged surface of 3-(trimethoxysilyl) propylamin modified sio2@fe3o4. the resulting cmpei-ni2+@sio2@fe3o4 composite nanoparticles displayed well-uniform structure and high magnetic responsiveness. hexa his-tagged peptides and purified his-tagged recombinant retinoid x receptor alpha were chosen as the model samples to evaluate the adsorption, capacity, and reusability of the composite nanoparticles. the results demonstrated the cmpei-ni2+@sio2@fe3o4 nanoparticles possessed rapid adsorption, large capacity, and good recyclability. the obtained nanoparticles were further used to purify his-tagged protein in practical environment. it was found that the nanoparticles could selectively capture his-tagged recombinant retinoid x receptor protein from complex cell lysate. owing to its easy synthesis, large binding capacity, and good reusability, the prepared cmpei-ni2+@sio2@fe3o4 magnetic nanoparticles have great potential for application in biotechnological fields. |
WOS关键词 | SELECTIVE REMOVAL ; SEPARATION ; ADSORPTION ; EXPRESSION ; IMMOBILIZATION ; MICROSPHERES ; EXTRACTION ; COMPOSITE ; SILICA |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
语种 | 英语 |
WOS记录号 | WOS:000457651000013 |
出版者 | WILEY-V C H VERLAG GMBH |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2372814 |
专题 | 大连化学物理研究所 |
通讯作者 | Shi, Xianzhe |
作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Chang, Mengmeng,Qin, Qian,Wang, Bohong,et al. Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of his-tagged protein[J]. Journal of separation science,2019,42(3):744-753. |
APA | Chang, Mengmeng.,Qin, Qian.,Wang, Bohong.,Xia, Tian.,Lv, Wangjie.,...&Xu, Guowang.(2019).Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of his-tagged protein.Journal of separation science,42(3),744-753. |
MLA | Chang, Mengmeng,et al."Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of his-tagged protein".Journal of separation science 42.3(2019):744-753. |
入库方式: iSwitch采集
来源:大连化学物理研究所
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