Stereoselective catalysis controlled by a native leucine or variant isoleucine wing-gatekeeper in 2-haloacid dehalogenase
文献类型：期刊论文
| 作者 | Feng, Yanbin1; Wang, Yayue1,2; Chu, Huiying3; Fan, Yan1,4; Cao, Xupeng1; Liu, Yinghui1; Li, Guohui3; Xue, Song1 |
| 刊名 | Febs letters
 |
| 出版日期 | 2019-02-01 |
| 卷号 | 593期号:3页码:308-318 |
| ISSN号 | 1873-3468 |
| 关键词 | Biocatalyst Enzyme Molecular dynamics Protein engineering Stereoselectivity |
| DOI | 10.1002/1873-3468.13309 |
| 通讯作者 | Li, guohui(ghli@dicp.ac.cn) ; Xue, song(xuesong@dicp.ac.cn) |
| 英文摘要 | Comprehensively understanding enzymatic stereoselectivity will assist in the creation of new enzymes for producing optically pure compounds for chemical applications. the essential features for selecting enantiomers are controlled by particular residues or regions of the enzymes. we report a stereoselective mechanism in the d-2-haloacid dehalogenase hadd aj1, in which l288 is identified as a gatekeeper in the access channel that strictly recognizes d-enantiomers. mutagenesis of l288 to isoleucine (i) enlarges the size of the channel and allows the enzyme to accommodate l-enantiomers. furthermore, the wing flip of i288 induces hydrophobic interactions with the l-enantiomer and directly affects the catalytic efficiency. the results illustrate the dynamic catalytic mechanisms of leu-ile gatekeepers and provide knowledge for unveiling the basis of stereospecificity in biocatalysts. |
| WOS关键词 | DL-2-HALOACID DEHALOGENASE ; HALOALKANE-DEHALOGENASE ; CRYSTAL-STRUCTURE ; ACTIVE-SITE ; ENANTIOSELECTIVITY ; ENZYMES ; REVEAL ; TUNNEL |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000458335400005 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2372834 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Li, Guohui; Xue, Song |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Shangqiu Normal Univ, Sch Biol & Food Sci, Shangqiu, Peoples R China 3.Chinese Acad Sci, Lab Mol Modeling & Design, State Key Lab Mol React Dynam, Dalian Inst Chem Phys, Dalian, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Yanbin,Wang, Yayue,Chu, Huiying,et al. Stereoselective catalysis controlled by a native leucine or variant isoleucine wing-gatekeeper in 2-haloacid dehalogenase[J]. Febs letters,2019,593(3):308-318. |
| APA | Feng, Yanbin.,Wang, Yayue.,Chu, Huiying.,Fan, Yan.,Cao, Xupeng.,...&Xue, Song.(2019).Stereoselective catalysis controlled by a native leucine or variant isoleucine wing-gatekeeper in 2-haloacid dehalogenase.Febs letters,593(3),308-318. |
| MLA | Feng, Yanbin,et al."Stereoselective catalysis controlled by a native leucine or variant isoleucine wing-gatekeeper in 2-haloacid dehalogenase".Febs letters 593.3(2019):308-318. |
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来源：大连化学物理研究所
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