Enhancement of torularhodin production in rhodosporidium toruloides by agrobacterium tumefaciens-mediated transformation and culture condition optimization
文献类型：期刊论文
| 作者 | Bao, Ruiqi1; Gao, Ning2; Lv, Jing1; Ji, Chaofan1; Liang, Huipeng1; Li, Shengjie1; Yu, Chenxu3; Wang, Zhenyu1; Lin, Xinping1 |
| 刊名 | Journal of agricultural and food chemistry
 |
| 出版日期 | 2019-01-30 |
| 卷号 | 67期号:4页码:1156-1164 |
| 关键词 | Rhodosporidium toruloides Torularhodin Agrobacterium tumefaciens-mediated transformation Response surface methodology Selective production |
| ISSN号 | 0021-8561 |
| DOI | 10.1021/acs.jafc.8b04667 |
| 通讯作者 | Wang, zhenyu(wangzy0506@outlook.com) ; Lin, xinping(yingchaer@163.com) |
| 英文摘要 | Nine transformants of rhodosporidium toruloides with significant changes in the carotenoid profile were obtained by agrobacterium tumefaciens-mediated transformation, including a white, three red, and four yellow mutants. a red mutant al-15-brq that showed a high torularhodin production was selected for culture condition optimization. results indicated that the torularhodin yield was boosted with glucose as the carbon source, at a carbon/nitrogen ratio of 22, a loading volume of 75 ml, and 28 degrees c. the torularhodin yield of 21.3 mg/l consisting of 94.4% total carotenoids was obtained by box-behnken design experiments. the torularhodin yield was 17.0-fold higher than that of the wild type, with time shortened from 9 to 3 days. this study reports an effective strategy for improving torularhodin production and provides a candidate r toruloides strain for highly selective production of torularhodin. |
| WOS关键词 | YEAST RHODOTORULA-GLUTINIS ; CAROTENOID PRODUCTION ; BETA-CAROTENE ; LIPID PRODUCTION ; C/N RATIO ; GLYCEROL ; GROWTH ; MUTANT ; TEMPERATURE ; PROFILES |
| WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
| WOS类目 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000457815800014 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2372841 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Wang, Zhenyu; Lin, Xinping |
| 作者单位 | 1.Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, 1 Qinggongyuan, Dalian 116034, Liaoning, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China 3.Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA |
| 推荐引用方式 GB/T 7714 | Bao, Ruiqi,Gao, Ning,Lv, Jing,et al. Enhancement of torularhodin production in rhodosporidium toruloides by agrobacterium tumefaciens-mediated transformation and culture condition optimization[J]. Journal of agricultural and food chemistry,2019,67(4):1156-1164. |
| APA | Bao, Ruiqi.,Gao, Ning.,Lv, Jing.,Ji, Chaofan.,Liang, Huipeng.,...&Lin, Xinping.(2019).Enhancement of torularhodin production in rhodosporidium toruloides by agrobacterium tumefaciens-mediated transformation and culture condition optimization.Journal of agricultural and food chemistry,67(4),1156-1164. |
| MLA | Bao, Ruiqi,et al."Enhancement of torularhodin production in rhodosporidium toruloides by agrobacterium tumefaciens-mediated transformation and culture condition optimization".Journal of agricultural and food chemistry 67.4(2019):1156-1164. |
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来源：大连化学物理研究所
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