Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants
文献类型：期刊论文
| 作者 | Du, Nana1,2; Si, Wei1; Liu, Yanfang1; Guo, Zhimou1; Zhou, Weijia1,2; Zhou, Han1; Liang, Xinmiao1; Wang, Chaoran1 |
| 刊名 | Journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2019-01-15 |
| 卷号 | 1105页码:148-155 |
| 关键词 | Alkaloid enrichment Electrostatic repulsion Reversed-phase/strong anion-exchange Scopolia tangutica Mixed-mode stationary phase Class separation |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2018.12.024 |
| 通讯作者 | Liu, yanfang(liuyanfang@dicp.ac.cn) ; Liang, xinmiao(liangxm@dicp.ac.cn) |
| 英文摘要 | Since the content of alkaloids is usually low in plants and they are easily co-eluted with other constituents, enrichment of alkaloids is essential in the discovery of bioactive lead compounds from natural products. in this paper, an easy spe enrichment method was developed in a buffer-free solvent system based on electrostatic repulsion mechanism. the feasibility of the new method was verified by successful enrichment of alkaloids from scopolia tangutica (s. tangutica) with an optimized eluting condition. then this developed method was applied to other representative plants in different families, including przewalskia tangutica and peganum harmala l, lycoris radiata and menispermum dauricum dc, which enlarged the scope of applicability. additionally, the new spe procedure avoided possible structural change destruction caused by ph change. simple solvent system, including formic acid (fa) and methanol, would benefit subsequent mass analysis, quantity determination and bioactivity screening, and so on. |
| WOS关键词 | LIQUID-CHROMATOGRAPHY ; ACONITUM ALKALOIDS ; ALZHEIMERS-DISEASE ; PHASE ; EXTRACTION ; SEPARATION ; SURFACE ; COLUMN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000456893100017 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2372875 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Liu, Yanfang; Liang, Xinmiao |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Nana,Si, Wei,Liu, Yanfang,et al. Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants[J]. Journal of chromatography b-analytical technologies in the biomedical and life sciences,2019,1105:148-155. |
| APA | Du, Nana.,Si, Wei.,Liu, Yanfang.,Guo, Zhimou.,Zhou, Weijia.,...&Wang, Chaoran.(2019).Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants.Journal of chromatography b-analytical technologies in the biomedical and life sciences,1105,148-155. |
| MLA | Du, Nana,et al."Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants".Journal of chromatography b-analytical technologies in the biomedical and life sciences 1105(2019):148-155. |
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来源：大连化学物理研究所
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