Ultrasensitive ambient mass spectrometry immunoassays: multiplexed detection of proteins in serum and on cell surfaces
文献类型：期刊论文
| 作者 | Xu, Shuting; Ma, Wen; Bai, Yu; Liu, Huwei |
| 刊名 | Journal of the american chemical society
 |
| 出版日期 | 2019-01-09 |
| 卷号 | 141期号:1页码:72-75 |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/jacs.8b10853 |
| 通讯作者 | Bai, yu(yu.bai@pku.edu.cn) |
| 英文摘要 | The sensitive and accurate determination of multiple protein biomarkers for clinical diagnosis is in high demand. here, an ambient mass spectrometry immunoassay platform that possesses advantages of high sensitivity, multiplexed quantitation, low sample consumption and convenient operation was established. a series of extensible rhodamine-based mass tags that ensured quantification of multiple proteins with ultrahigh sensitivity through two-stage signal amplification were developed. thrombin was detected at zeptomole sensitivity in 2 mu l serum/plasma, as was free cancer antigen 125 (ca125). three protein biomarkers (ca125, carcinoembryonic antigen, and epithelial cell adhesion molecule) were simultaneously detected in situ on ca. 20 cells. this platform is promising for multiple protein detection in a single drop of sample or at the single-cell scale for clinical diagnosis and therapy. |
| WOS关键词 | CANCER ; EXPRESSION ; BREAST ; MUCINS ; CA125 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000455561800013 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2372899 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Bai, Yu |
| 作者单位 | Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Shuting,Ma, Wen,Bai, Yu,et al. Ultrasensitive ambient mass spectrometry immunoassays: multiplexed detection of proteins in serum and on cell surfaces[J]. Journal of the american chemical society,2019,141(1):72-75. |
| APA | Xu, Shuting,Ma, Wen,Bai, Yu,&Liu, Huwei.(2019).Ultrasensitive ambient mass spectrometry immunoassays: multiplexed detection of proteins in serum and on cell surfaces.Journal of the american chemical society,141(1),72-75. |
| MLA | Xu, Shuting,et al."Ultrasensitive ambient mass spectrometry immunoassays: multiplexed detection of proteins in serum and on cell surfaces".Journal of the american chemical society 141.1(2019):72-75. |
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来源：大连化学物理研究所
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