A biomimetic human gut-on-a-chip for modeling drug metabolism in intestine
文献类型：期刊论文
| 作者 | Guo, Yaqiong1,2; Li, Zhongyu1; Su, Wentao1; Wang, Li1; Zhu, Yujuan1,2; Qin, Jianhua1,2,3 |
| 刊名 | Artificial organs
 |
| 出版日期 | 2018-12-01 |
| 卷号 | 42期号:12页码:1196-1205 |
| 关键词 | Gut-on-a-chip Caco-2 Intestinal metabolism Biomimetic Drug metabolism |
| ISSN号 | 0160-564X |
| DOI | 10.1111/aor.13163 |
| 通讯作者 | Qin, jianhua(jhqin@dicp.ac.cn) |
| 英文摘要 | Drug metabolism in the intestine is considered to substantially contribute to the overall first-pass metabolism, which has been neglected for a long time. it is highly desirable to develop a reliable model to evaluate drug metabolism in the intestine in vitro. in this work, we made the first attempt to develop a biomimetic human gut-on-a-chip for modeling drug metabolism in intestine. in this chip, constant flow, together with porous nitrocellulose membrane and collagen i, mimics an in vivo-like intestinal microenvironment. the caco-2 cells grown in the chip formed a compact intestinal epithelial layer with continuous expression of the tight junction protein, zo-1. furthermore, higher gene expression of villin, sucrase-isomaltase, and alkaline phosphatase demonstrated that cells in the biomimetic human gut-on-a-chip device were more mature with near-physiological functions compared to the control on planar substrate. in particular, cellular metabolic activity was assessed on different substrates, indicating higher metabolic efficiency of ifosfamide and verapamil in the biomimetic human gut-on-a-chip model. taken together, our results suggested that this biomimetic human gut-on-a-chip promoted the differentiation of intestinal cells with enhanced functionality by creating a biomimetic 3d microenvironment in vitro. it might offer a bioactive, low-cost, and flexible in vitro platform for studies on intestinal metabolism as well as preclinical drug development. |
| WOS关键词 | CELL-LINE CACO-2 ; CARDIAC TISSUES ; PERMEABILITY ; VERAPAMIL ; ABSORPTION ; INDUCTION ; SYSTEM ; VILLIN |
| WOS研究方向 | Engineering ; Transplantation |
| WOS类目 | Engineering, Biomedical ; Transplantation |
| 语种 | 英语 |
| WOS记录号 | WOS:000454127100016 |
| 出版者 | WILEY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2372988 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Qin, Jianhua |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Sch Future Technol, Beijing, Peoples R China 3.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Yaqiong,Li, Zhongyu,Su, Wentao,et al. A biomimetic human gut-on-a-chip for modeling drug metabolism in intestine[J]. Artificial organs,2018,42(12):1196-1205. |
| APA | Guo, Yaqiong,Li, Zhongyu,Su, Wentao,Wang, Li,Zhu, Yujuan,&Qin, Jianhua.(2018).A biomimetic human gut-on-a-chip for modeling drug metabolism in intestine.Artificial organs,42(12),1196-1205. |
| MLA | Guo, Yaqiong,et al."A biomimetic human gut-on-a-chip for modeling drug metabolism in intestine".Artificial organs 42.12(2018):1196-1205. |
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来源：大连化学物理研究所
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