Loop permutation affects the topology and stability of g-quadruplexes
文献类型：期刊论文
| 作者 | Cheng, Mingpan1,2; Cheng, Yu1,2; Hao, Jingya1,2; Jia, Guoqing1; Zhou, Jun3; Mergny, Jean-Louis3,4,5; Li, Can1 |
| 刊名 | Nucleic acids research
 |
| 出版日期 | 2018-10-12 |
| 卷号 | 46期号:18页码:9264-9275 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gky757 |
| 通讯作者 | Mergny, jean-louis(jean-louis.mergny@inserm.fr) ; Li, can(canli@dicp.ac.cn) |
| 英文摘要 | G-quadruplexes are unusual dna and rna secondary structures ubiquitous in a variety of organisms including vertebrates, plants, viruses and bacteria. the folding topology and stability of intramolecular g-quadruplexes are determined to a large extent by their loops. loop permutation is defined as swapping two or three of these regions so that intramolecular g-quadruplexes only differ in the sequential order of their loops. over the past two decades, both length and base composition of loops have been studied extensively, but a systematic study on the effect of loop permutation has been missing. in the present work, 99 sequences from 21 groups with different loop permutations were tested. to our surprise, both conformation and thermal stability are greatly dependent on loop permutation. loop permutation actually matters as much as loop length and base composition on g-quadruplex folding, with effects on t-m as high as 17 degrees c. sequences containing a longer central loop have a high propensity to adopt a stable non-parallel topology. conversely, sequences containing a short central loop tend to form a parallel topology of lower stability. in addition, over half of interrogated sequences were found in the genomes of diverse organisms, implicating their potential regulatory roles in the genome or as therapeutic targets. this study illustrates the structural roles of loops in g-quadruplex folding and should help to establish rules to predict the folding pattern and stability of g-quadruplexes. |
| WOS关键词 | TELOMERIC G-QUADRUPLEX ; DNA G-QUADRUPLEX ; SINGLE-BASE LOOPS ; K+ SOLUTION ; ANTI-HIV ; SEQUENCE ; LENGTH ; PROMOTER ; INSTABILITY ; INSIGHTS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000450953200008 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373055 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Mergny, Jean-Louis; Li, Can |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China 4.Univ Bordeaux, CNRS, INSERM, ARNA Lab,IECB,U1212,UMR5320, F-33607 Pessac, France 5.CAS, Vvi, Inst Biophys, Kralovopolska 135, Brno 61265, Czech Republic |
| 推荐引用方式 GB/T 7714 | Cheng, Mingpan,Cheng, Yu,Hao, Jingya,et al. Loop permutation affects the topology and stability of g-quadruplexes[J]. Nucleic acids research,2018,46(18):9264-9275. |
| APA | Cheng, Mingpan.,Cheng, Yu.,Hao, Jingya.,Jia, Guoqing.,Zhou, Jun.,...&Li, Can.(2018).Loop permutation affects the topology and stability of g-quadruplexes.Nucleic acids research,46(18),9264-9275. |
| MLA | Cheng, Mingpan,et al."Loop permutation affects the topology and stability of g-quadruplexes".Nucleic acids research 46.18(2018):9264-9275. |
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来源：大连化学物理研究所
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