Chemoenzymatic approach for the proteomics analysis of mucin-type core-1 o-glycosylation in human serum
文献类型：期刊论文
| 作者 | You, Xin1,2; Yao, Yating1,2; Mao, Jiawei1,2; Qin, Hongqiang1,2; Liang, Xinmiao1,2; Wang, Liming3; Ye, Mingliang1,2 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2018-11-06 |
| 卷号 | 90期号:21页码:12714-12722 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b02993 |
| 通讯作者 | Ye, mingliang(mingliang@dicp.ac.cn) |
| 英文摘要 | Human serum is a complex body fluid that contains various n-linked and o-linked glycoproteins. compared with n-linked glycoproteins, the serum o-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance. herein, we presented a novel chemoenzymatic method to analyze core-1 type of o-galnacylation in human serum. in this approach, the tryptic digest of serum was first subjected to pngase f treatment to release the n-glycan and was then treated with strong acid to release sialic acid residues from mucin-type o-glycans. in this way, the internal gal/galnac residues were exposed and were oxidized by the galactose oxidase to carry the aldehyde groups. the oxidized o-galnacylated peptides were then captured by hydrazide beads and eluted with methoxylamine for lc-ms/ms analysis. the de-n-deglycosylation decreased the abundance of n-glycopeptides, the desialylation simplified the o-glycans and the enzymatic oxidization conferred the enrichment specificity. we have demonstrated that this method was fitted to analyze o-galnacylated peptides with high confidence. this method was applied to analyze human serum, which resulted in the identification of 59 o-galnac modified peptide sequences corresponding to 38 glycoproteins from 50 mu l of serum. this method is expected to have broad applications in the analysis of o-glycoproteome. |
| WOS关键词 | INTERACTION LIQUID-CHROMATOGRAPHY ; LARGE GENE LISTS ; MASS-SPECTROMETRY ; ENRICHMENT ; IDENTIFICATION ; GLYCOPEPTIDES ; PROTEINS ; STRATEGY ; CELLS ; GLYCOPROTEOME |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000449722500054 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373071 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Ye, Mingliang |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Med Univ, Hosp 2, Dept Surg, Div Hepatobiliary & Pancreat Surg, Dalian 116023, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Xin,Yao, Yating,Mao, Jiawei,et al. Chemoenzymatic approach for the proteomics analysis of mucin-type core-1 o-glycosylation in human serum[J]. Analytical chemistry,2018,90(21):12714-12722. |
| APA | You, Xin.,Yao, Yating.,Mao, Jiawei.,Qin, Hongqiang.,Liang, Xinmiao.,...&Ye, Mingliang.(2018).Chemoenzymatic approach for the proteomics analysis of mucin-type core-1 o-glycosylation in human serum.Analytical chemistry,90(21),12714-12722. |
| MLA | You, Xin,et al."Chemoenzymatic approach for the proteomics analysis of mucin-type core-1 o-glycosylation in human serum".Analytical chemistry 90.21(2018):12714-12722. |
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