Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived chlamydomonas reinhardtii
文献类型：期刊论文
| 作者 | Yang, Miao1,2,3; Meng, Yingying1,4; Chu, Yadong1; Fan, Yan1,2; Cao, Xupeng1; Xue, Song1; Chi, Zhanyou3 |
| 刊名 | Biochimica et biophysica acta-molecular and cell biology of lipids
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| 出版日期 | 2018-12-01 |
| 卷号 | 1863期号:12页码:1478-1487 |
| 关键词 | Chlamydomonas Chloroplast Extra-chloroplastidic tag accumulation Membrane glycerolipids Short-term nitrogen starvation Subcellular glycerolipidomics |
| ISSN号 | 1388-1981 |
| DOI | 10.1016/j.bbalip.2018.09.009 |
| 通讯作者 | Xue, song(xuesong@dicp.ac.cn) |
| 英文摘要 | In microalgae, triacylglycerol (tag) biosynthesis occurs by parallel pathways involving both the chloroplast and endoplasmic reticulum. a better understanding of contribution of each pathway to tag assembly facilitates enhanced tag production via rational genetic engineering of microalgae. here, using a uplc-ms(/ms) coupled with tlc-gc-based lipidomic platform, the early response of the major glycerolipids to nitrogen stress was analyzed at both the cellular and chloroplastidic levels in the model green alga chlamydomonas reinhardtii. subcellular lipidomic analysis demonstrated that tag was accumulated exclusively outside the chloroplast, and remained unaltered inside the chloroplast after 4 h of nitrogen starvation. this study ascertained the existence of the glycolipid, digalactosyldiacylglycerol (dgdg), outside the chloroplast and the betaine lipid, diacylglycerol-n,n,n-trimethylhomoserine (dgts), inside the chloroplast. the newly synthesized dgdg and dgts prominently increased at the extra-chloroplastidic compartments and served as the major precursors for tag biosynthesis. in particular, dgdg contributed to the extra-chloroplastidic tag assembly in form of diacylglycerol (dag) and dgts in form of acyl groups. the chloroplastidic membrane lipid, monogalactosyldiacylglycerol (mgdg), was proposed to primarily offer dag for tag formation outside the chloroplast. this study provides valuable insights into the subcellular glycerolipidomics and unveils the acyl flux into the extra-chloroplastidic tag in microalgae. |
| WOS关键词 | ACID BETA-OXIDATION ; LIPID-METABOLISM ; NANNOCHLOROPSIS-OCEANICA ; MEMBRANE PHOSPHOLIPIDS ; PHOSPHATE DEPRIVATION ; PLASMA-MEMBRANE ; BIOSYNTHESIS ; MICROALGAE ; ACYLTRANSFERASE ; IDENTIFICATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000449898800005 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373072 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Xue, Song |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 4.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Miao,Meng, Yingying,Chu, Yadong,et al. Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived chlamydomonas reinhardtii[J]. Biochimica et biophysica acta-molecular and cell biology of lipids,2018,1863(12):1478-1487. |
| APA | Yang, Miao.,Meng, Yingying.,Chu, Yadong.,Fan, Yan.,Cao, Xupeng.,...&Chi, Zhanyou.(2018).Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived chlamydomonas reinhardtii.Biochimica et biophysica acta-molecular and cell biology of lipids,1863(12),1478-1487. |
| MLA | Yang, Miao,et al."Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived chlamydomonas reinhardtii".Biochimica et biophysica acta-molecular and cell biology of lipids 1863.12(2018):1478-1487. |
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来源：大连化学物理研究所
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