中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Saikosaponin d from radix bupleuri suppresses triple-negative breast cancer cell growth by targeting beta-catenin signaling

文献类型:期刊论文

作者Wang, Jixia1; Qi, Huan1; Zhang, Xiuli1,4; Si, Wei1; Xu, Fangfang1; Hou, Tao1; Zhou, Han1; Wang, Anhui2; Li, Guohui2; Liu, Yanfang1
刊名Biomedicine & pharmacotherapy
出版日期2018-12-01
卷号108页码:724-733
ISSN号0753-3322
关键词Triple-negative breast cancer Saikosaponin d Radix bupleuri Wnt/beta-catenin
DOI10.1016/j.biopha.2018.09.038
通讯作者Zhang, xiuli(zhangxiuli@dicp.ac.cn) ; Piao, hai-long(hpiao@dicp.ac.cn) ; Liang, xinmiao(liangxm@dicp.ac.cn)
英文摘要Background: triple-negative breast cancer (tnbc) is one of the most aggressive and poor prognosis breast cancers. currently, chemotherapy with conventional cytotoxic agents is the only available option to treat tnbc. hence, we identified new therapeutic agents against tnbc from traditional chinese medicine radix bupleuri and unveiled the molecule mechanism of anti-tnbc effects. methods: multi-component bioactivity and structure-guided methods were used to identify the most effective anti-tnbc compound saikosaponin d (ssd) from radix bupleuri. cell viability and apoptosis assays were employed to demonstrate the effect of ssd on the proliferation and apoptosis of tnbc cells. dynamic mass redistribution assay, topflash assay, western blotting, and special agonist were applied to dissect the potential molecular mechanisms of ssd. results: we screened twenty fractions in radix bupleuri and identified ssd as the most effective component to inhibit the proliferation of tnbc cells. investigating the interaction of ssd with the frequently overexpressed targets in tnbc led to the identification that it markedly suppressed wnt/beta-catenin signaling, but did not act on epidermal growth factor receptor and neurotensin receptor-1. moreover, we demonstrated that ssd significantly repressed beta-catenin and its downstream target genes, resulting in tnbc cell apoptosis. specifically, docking of ssd to the crystal structure of beta-catenin suggested that ssd interacted with beta-catenin via hydrogen bonds and hydrophobic interaction. conclusion: we identified the most effective component ssd from radix bupleuri in inhibiting the proliferation of tnbc cells by targeting beta-catenin signaling. given the important role of wnt/beta-catenin signaling in breast cancer, ssd may present an opportunity to discover new therapeutics for the treatment of tnbc.
WOS关键词D INHIBITS PROLIFERATION ; FACTOR RECEPTOR ; NEUROTENSIN RECEPTOR-1 ; CARCINOMA CELLS ; PROSTATE-CANCER ; MECHANISM ; APOPTOSIS ; CYCLOOXYGENASE-2 ; THERAPIES ; EXTRACTS
WOS研究方向Research & Experimental Medicine ; Pharmacology & Pharmacy
WOS类目Medicine, Research & Experimental ; Pharmacology & Pharmacy
语种英语
出版者ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
WOS记录号WOS:000450101800083
URI标识http://www.irgrid.ac.cn/handle/1471x/2373084
专题大连化学物理研究所
通讯作者Zhang, Xiuli; Piao, Hai-long; Liang, Xinmiao
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Mol Modeling & Design, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
3.Corning, Biochem Technol, Div Sci & Technol, Corning, NY 14831 USA
4.Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226019, Peoples R China
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GB/T 7714
Wang, Jixia,Qi, Huan,Zhang, Xiuli,et al. Saikosaponin d from radix bupleuri suppresses triple-negative breast cancer cell growth by targeting beta-catenin signaling[J]. Biomedicine & pharmacotherapy,2018,108:724-733.
APA Wang, Jixia.,Qi, Huan.,Zhang, Xiuli.,Si, Wei.,Xu, Fangfang.,...&Liang, Xinmiao.(2018).Saikosaponin d from radix bupleuri suppresses triple-negative breast cancer cell growth by targeting beta-catenin signaling.Biomedicine & pharmacotherapy,108,724-733.
MLA Wang, Jixia,et al."Saikosaponin d from radix bupleuri suppresses triple-negative breast cancer cell growth by targeting beta-catenin signaling".Biomedicine & pharmacotherapy 108(2018):724-733.

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来源:大连化学物理研究所

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