Saikosaponin d from radix bupleuri suppresses triple-negative breast cancer cell growth by targeting beta-catenin signaling
文献类型：期刊论文
| 作者 | Wang, Jixia1; Qi, Huan1; Zhang, Xiuli1,4; Si, Wei1; Xu, Fangfang1; Hou, Tao1; Zhou, Han1; Wang, Anhui2; Li, Guohui2; Liu, Yanfang1 |
| 刊名 | Biomedicine & pharmacotherapy
 |
| 出版日期 | 2018-12-01 |
| 卷号 | 108页码:724-733 |
| 关键词 | Triple-negative breast cancer Saikosaponin d Radix bupleuri Wnt/beta-catenin |
| ISSN号 | 0753-3322 |
| DOI | 10.1016/j.biopha.2018.09.038 |
| 通讯作者 | Zhang, xiuli(zhangxiuli@dicp.ac.cn) ; Piao, hai-long(hpiao@dicp.ac.cn) ; Liang, xinmiao(liangxm@dicp.ac.cn) |
| 英文摘要 | Background: triple-negative breast cancer (tnbc) is one of the most aggressive and poor prognosis breast cancers. currently, chemotherapy with conventional cytotoxic agents is the only available option to treat tnbc. hence, we identified new therapeutic agents against tnbc from traditional chinese medicine radix bupleuri and unveiled the molecule mechanism of anti-tnbc effects. methods: multi-component bioactivity and structure-guided methods were used to identify the most effective anti-tnbc compound saikosaponin d (ssd) from radix bupleuri. cell viability and apoptosis assays were employed to demonstrate the effect of ssd on the proliferation and apoptosis of tnbc cells. dynamic mass redistribution assay, topflash assay, western blotting, and special agonist were applied to dissect the potential molecular mechanisms of ssd. results: we screened twenty fractions in radix bupleuri and identified ssd as the most effective component to inhibit the proliferation of tnbc cells. investigating the interaction of ssd with the frequently overexpressed targets in tnbc led to the identification that it markedly suppressed wnt/beta-catenin signaling, but did not act on epidermal growth factor receptor and neurotensin receptor-1. moreover, we demonstrated that ssd significantly repressed beta-catenin and its downstream target genes, resulting in tnbc cell apoptosis. specifically, docking of ssd to the crystal structure of beta-catenin suggested that ssd interacted with beta-catenin via hydrogen bonds and hydrophobic interaction. conclusion: we identified the most effective component ssd from radix bupleuri in inhibiting the proliferation of tnbc cells by targeting beta-catenin signaling. given the important role of wnt/beta-catenin signaling in breast cancer, ssd may present an opportunity to discover new therapeutics for the treatment of tnbc. |
| WOS关键词 | D INHIBITS PROLIFERATION ; FACTOR RECEPTOR ; NEUROTENSIN RECEPTOR-1 ; CARCINOMA CELLS ; PROSTATE-CANCER ; MECHANISM ; APOPTOSIS ; CYCLOOXYGENASE-2 ; THERAPIES ; EXTRACTS |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| WOS类目 | Medicine, Research & Experimental ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000450101800083 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373084 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Zhang, Xiuli; Piao, Hai-long; Liang, Xinmiao |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Mol Modeling & Design, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China 3.Corning, Biochem Technol, Div Sci & Technol, Corning, NY 14831 USA 4.Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226019, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jixia,Qi, Huan,Zhang, Xiuli,et al. Saikosaponin d from radix bupleuri suppresses triple-negative breast cancer cell growth by targeting beta-catenin signaling[J]. Biomedicine & pharmacotherapy,2018,108:724-733. |
| APA | Wang, Jixia.,Qi, Huan.,Zhang, Xiuli.,Si, Wei.,Xu, Fangfang.,...&Liang, Xinmiao.(2018).Saikosaponin d from radix bupleuri suppresses triple-negative breast cancer cell growth by targeting beta-catenin signaling.Biomedicine & pharmacotherapy,108,724-733. |
| MLA | Wang, Jixia,et al."Saikosaponin d from radix bupleuri suppresses triple-negative breast cancer cell growth by targeting beta-catenin signaling".Biomedicine & pharmacotherapy 108(2018):724-733. |
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来源：大连化学物理研究所
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