Measurement of carcinoembryonic antigen in clinical serum samples using a centrifugal microfluidic device
文献类型：期刊论文
| 作者 | Gao, Zhigang1; Chen, Zongzheng2; Deng, Jiu1; Li, Xiaorui1; Qu, Yueyang1; Xu, Lingling1; Luo, Yong1; Lu, Yao3; Liu, Tingjiao4; Zhao, Weijie1 |
| 刊名 | Micromachines
 |
| 出版日期 | 2018-09-01 |
| 卷号 | 9期号:9页码:10 |
| ISSN号 | 2072-666X |
| 关键词 | Centrifugal microfluidic device Cea detection Density medium Fluorescent chemiluminescence |
| DOI | 10.3390/mi9090470 |
| 通讯作者 | Luo, yong(yluo@dlut.edu.cn) ; Zhao, weijie(zyzhao@dlut.edu.cn) |
| 英文摘要 | Carcinoembryonic antigen (cea) is a broad-spectrum tumor marker used in clinical applications. the primarily clinical method for measuring cea is based on chemiluminescence in serum during enzyme-linked immunosorbent assays (elisa) in 96-well plates. however, this multi-step process requires large and expensive instruments, and takes a long time. in this study, a high-throughput centrifugal microfluidic device was developed for detecting cea in serum without the need for cumbersome washing steps normally used in immunoreactions. this centrifugal microdevice contains 14 identical pencil-like units, and the cea molecules are separated from the bulk serum for subsequent immunofluorescence detection using density gradient centrifugation in each unit simultaneously. to determine the optimal conditions for cea detection in serum, the effects of the density of the medium, rotation speed, and spin duration were investigated. the measured values from 34 clinical serum samples using this high-throughput centrifugal microfluidic device showed good agreement with the known values (average relative error = 9.22%). these results indicate that the high-throughput centrifugal microfluidic device could provide an alternative approach for replacing the classical method for cea detection in clinical serum samples. |
| WOS关键词 | CARCINO EMBRYONIC ANTIGEN ; BREAST-CANCER PATIENTS ; COLORECTAL-CANCER ; IMMUNOASSAY ; CEA ; ADENOCARCINOMA ; CHEMOTHERAPY ; CA-19-9 |
| WOS研究方向 | Science & Technology - Other Topics ; Instruments & Instrumentation |
| WOS类目 | Nanoscience & Nanotechnology ; Instruments & Instrumentation |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000448399300052 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373141 |
| 专题 | 大连化学物理研究所 |
| 通讯作者 | Luo, Yong; Zhao, Weijie |
| 作者单位 | 1.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, Dalian 116024, Peoples R China 2.Jinan Univ, Integrated Chinese & Western Med Postdoctoral Res, Guangzhou 510632, Guangdong, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 4.Dalian Med Univ, Coll Stomatol, Dalian 116024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Zhigang,Chen, Zongzheng,Deng, Jiu,et al. Measurement of carcinoembryonic antigen in clinical serum samples using a centrifugal microfluidic device[J]. Micromachines,2018,9(9):10. |
| APA | Gao, Zhigang.,Chen, Zongzheng.,Deng, Jiu.,Li, Xiaorui.,Qu, Yueyang.,...&Lin, Bingcheng.(2018).Measurement of carcinoembryonic antigen in clinical serum samples using a centrifugal microfluidic device.Micromachines,9(9),10. |
| MLA | Gao, Zhigang,et al."Measurement of carcinoembryonic antigen in clinical serum samples using a centrifugal microfluidic device".Micromachines 9.9(2018):10. |
入库方式： iSwitch采集
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。