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Zika virus infection induces rnai-mediated antiviral immunity in human neural progenitors and brain organoids

文献类型:期刊论文

AuthorXu, Yan-Peng1; Qiu, Yang2; Zhang, Boya3,4; Chen, Guilai3,4; Chen, Qi1; Wang, Miao2; Mo, Fan3,4; Xu, Jiuyue2,4; Wu, Jin5; Zhang, Rong-Rong1,6
SourceCell research
Issued Date2019-04-01
Volume29Issue:4Pages:265-273
ISSN1001-0602
DOI10.1038/s41422-019-0152-9
Corresponding AuthorHu, baoyang(byhu@ioz.ac.cn) ; Zhou, xi(zhouxi@wh.iov.cn) ; Qin, cheng-feng(qincf@bmi.ac.cn)
English AbstractThe re-emergence of zika virus (zikv) in the western hemisphere has resulted in global public health crisis since 2015. zikv preferentially infects and targets human neural progenitor cells (hnpcs) and causes fetal microcephaly upon maternal infection. hnpcs not only play critical roles during fetal brain development, but also persist in adult brain throughout life. yet the mechanism of innate antiviral immunity in hnpcs remains largely unknown. here, we show that zikv infection triggers the abundant production of virus-derived small interfering rnas in hnpcs, but not in the more differentiated progenies or somatic cells. ablation of key rnai machinery components significantly enhances zikv replication in hnpcs. furthermore, enoxacin, a broad-spectrum antibiotic that is known as an rnai enhancer, exerts potent anti-zikv activity in hnpcs and other rnai-competent cells. strikingly, enoxacin treatment completely prevents zikv infection and circumvents zikv-induced microcephalic phenotypes in brain organoid models that recapitulate human fetal brain development. our findings highlight the physiological importance of rnai-mediated antiviral immunity during the early stage of human brain development, uncovering a novel strategy to combat human congenital viral infections through enhancing rnai.
WOS KeywordSTEM-CELLS ; CEREBRAL ORGANOIDS ; INTERFERENCE ; IDENTIFICATION ; SUPPRESSION ; INHIBITION ; INDUCTION ; EMERGENCE
WOS Research AreaCell Biology
WOS SubjectCell Biology
Language英语
PublisherINST BIOCHEMISTRY & CELL BIOLOGY
WOS IDWOS:000462861400004
URIhttp://www.irgrid.ac.cn/handle/1471x/2373165
Collection武汉病毒研究所
Corresponding AuthorHu, Baoyang; Zhou, Xi; Qin, Cheng-Feng
Affiliation1.Acad Mil Med Sci, Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China
3.Chinese Acad Sci, State Key Lab Stem Cell & Reprod Biol, Inst Zool, Beijing 100101, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Natl Ctr Biomed Anal, Inst Basic Med Sci, State Key Lab Prote, Beijing 100850, Peoples R China
6.Anhui Med Univ, Sch Basic Sci, Hefei 230032, Anhui, Peoples R China
7.Wuhan Univ, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China
8.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Ctr Excellence Brain Sci & Intelligence Technol, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Xu, Yan-Peng,Qiu, Yang,Zhang, Boya,et al. Zika virus infection induces rnai-mediated antiviral immunity in human neural progenitors and brain organoids[J]. Cell research,2019,29(4):265-273.
APA Xu, Yan-Peng.,Qiu, Yang.,Zhang, Boya.,Chen, Guilai.,Chen, Qi.,...&Qin, Cheng-Feng.(2019).Zika virus infection induces rnai-mediated antiviral immunity in human neural progenitors and brain organoids.Cell research,29(4),265-273.
MLA Xu, Yan-Peng,et al."Zika virus infection induces rnai-mediated antiviral immunity in human neural progenitors and brain organoids".Cell research 29.4(2019):265-273.

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来源:武汉病毒研究所

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