N-6-methyladenosine modification and mettl3 modulate enterovirus 71 replication
文献类型：期刊论文
| 作者 | Hao, Haojie1,2; Hao, Sujuan1,2; Chen, Honghe1; Chen, Zhen1; Zhang, Yanfang1; Wang, Jun1; Wang, Hanzhong1; Zhang, Bo1; Qiu, Jianming3; Deng, Fei1 |
| 刊名 | Nucleic acids research
 |
| 出版日期 | 2019-01-10 |
| 卷号 | 47期号:1页码:362-374 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gky1007 |
| 通讯作者 | Deng, fei(df@wh.iov.cn) ; Guan, wuxiang(guanwx@wh.iov.cn) |
| 英文摘要 | N-6-methyladenosine (m(6)a) constitutes one of the most abundant internal rna modifications and is critical for rna metabolism and function. it has been previously reported that viral rna contains internal m(6)a modifications; however, only recently the function of m(6)a modification in viral rnas has been elucidated during infections of hiv, hepatitis c virus and zika virus. in the present study, we found that enterovirus 71 (ev71) rna undergoes m(6)a modification during viral infection, which alters the expression and localization of the methyltransferase and demethylase of m(6)a, and its binding proteins. moreover, knockdown of m(6)a methyltransferase resulted in decreased ev71 replication, whereas knockdown of the demethylase had the opposite effect. further study showed that the m(6)a binding proteins also participate in the regulation of viral replication. in particular, two m(6)a modification sites were identified in the viral genome, of which mutations resulted in decreased virus replication, suggesting that m(6)a modification plays an important role in ev71 replication. notably, we found that mettl3 interacted with viral rna-dependent rna polymerase 3d and induced enhanced sumoylation and ubiquitination of the 3d polymerase that boosted viral replication. taken together, our findings demonstrated that the host m(6)a modification complex interacts with viral proteins to modulate ev71 replication. |
| WOS关键词 | MESSENGER-RNA METHYLATION ; M(6)A RNA ; GENE-EXPRESSION ; NUCLEAR-RNA ; VIRUS ; N6-METHYLADENOSINE ; TRANSLATION ; M6A ; IDENTIFICATION ; METHYLOMES |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000462586700033 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373168 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Deng, Fei; Guan, Wuxiang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Kansas, Med Ctr, Dept Microbiol Mol Genet & Immunol, Kansas City, KS 66160 USA |
| 推荐引用方式 GB/T 7714 | Hao, Haojie,Hao, Sujuan,Chen, Honghe,et al. N-6-methyladenosine modification and mettl3 modulate enterovirus 71 replication[J]. Nucleic acids research,2019,47(1):362-374. |
| APA | Hao, Haojie.,Hao, Sujuan.,Chen, Honghe.,Chen, Zhen.,Zhang, Yanfang.,...&Guan, Wuxiang.(2019).N-6-methyladenosine modification and mettl3 modulate enterovirus 71 replication.Nucleic acids research,47(1),362-374. |
| MLA | Hao, Haojie,et al."N-6-methyladenosine modification and mettl3 modulate enterovirus 71 replication".Nucleic acids research 47.1(2019):362-374. |
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来源：武汉病毒研究所
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