Rapid direct drug susceptibility testing of mycobacterium tuberculosis based on culture droplet digital polymerase chain reaction
文献类型：期刊论文
| 作者 | Luo, J.1,2; Luo, M.3; Li, J.1; Yu, J.1; Yang, H.1; Yi, X.3; Chen, Y.3; Wei, H.1 |
| 刊名 | International journal of tuberculosis and lung disease
 |
| 出版日期 | 2019-02-01 |
| 卷号 | 23期号:2页码:219-+ |
| 关键词 | Droplet digital pcr Tb Drug susceptibility testing Rapid test |
| ISSN号 | 1027-3719 |
| DOI | 10.5588/ijtld.18.0182 |
| 通讯作者 | Chen, y.(yaokaichen@hotmail.com) ; Wei, h.(hpwei@wh.iov.cn) |
| 英文摘要 | Setting: early diagnosis and drug susceptibility testing are important for anti-tuberculosis treatment. objective: to develop a rapid method for detecting mycobacterium tuberculosis and drug susceptibility based on culture and droplet digital polymerase chain reaction (ddpcr). design: m. tuberculosis in 102 sputum samples was detected using ddpcr, xpert, quantitative pcr (qpcr) and mgit (tm) 960 (tm). the susceptibility of ddpcr-positive samples to rifampicin (rmp), isoniazid (inh) and streptomycin (sm) was tested by measuring changes in dna quantity over 4 days of culture. for comparison, susceptibility of mgit 960-positive samples was tested using the standard agar proportion method. results: the sensitivity and specificity of m. tuberculosis detection using ddpcr and mgit 960 were respectively 95.7% (95% ci 80.0-99.2) and 88.9% (95% ci 76.7-95.4). compared with agar proportion, the susceptibility of 44 specimens positive on culture-cddpcr showed sensitivity and specificity for rmp, inh and sm of respectively 83.3% (95% ci 50.9-97.1) and 90.6% (95% ci 73.8-97.6); 79.0% (95% ci 53.9-93.0) and 92% (95% ci 72.5-98.6); 94.1% (95% ci 69.2-99.7) and 92.6% (95% ci 74.3-98.7). conclusion: culture ddpcr could detect m. tuberculosis within 5 h and drug susceptibility within 4 days directly from sputum, which would greatly reduce the laboratory time needed for tuberculosis diagnosis. |
| WOS关键词 | DIAGNOSTIC METHODS ; ASSAY ; PCR ; DNA ; RESISTANCE ; SPUTUM |
| WOS研究方向 | Infectious Diseases ; Respiratory System |
| WOS类目 | Infectious Diseases ; Respiratory System |
| 语种 | 英语 |
| WOS记录号 | WOS:000459791400016 |
| 出版者 | INT UNION AGAINST TUBERCULOSIS LUNG DISEASE (I U A T L D) |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373174 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Y.; Wei, H. |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chongqing Publ Hlth Med Ctr, Chongqing 400036, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, J.,Luo, M.,Li, J.,et al. Rapid direct drug susceptibility testing of mycobacterium tuberculosis based on culture droplet digital polymerase chain reaction[J]. International journal of tuberculosis and lung disease,2019,23(2):219-+. |
| APA | Luo, J..,Luo, M..,Li, J..,Yu, J..,Yang, H..,...&Wei, H..(2019).Rapid direct drug susceptibility testing of mycobacterium tuberculosis based on culture droplet digital polymerase chain reaction.International journal of tuberculosis and lung disease,23(2),219-+. |
| MLA | Luo, J.,et al."Rapid direct drug susceptibility testing of mycobacterium tuberculosis based on culture droplet digital polymerase chain reaction".International journal of tuberculosis and lung disease 23.2(2019):219-+. |
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来源：武汉病毒研究所
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