Quantitative profiling of cd13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment
文献类型：期刊论文
| 作者 | Xi, Yan1,3,4; Wang, Dianbing2; Wang, Tingting2; Huang, Lin2; Zhang, Xian-En1,2,3 |
| 刊名 | Nanoscale
 |
| 出版日期 | 2019-01-28 |
| 卷号 | 11期号:4页码:1737-1744 |
| ISSN号 | 2040-3364 |
| DOI | 10.1039/c8nr06526h |
| 通讯作者 | Zhang, xian-en(zhangxe@ibp.ac.cn) |
| 英文摘要 | Quantitative profiling of membrane proteins on the cell surface is of great interest in tumor targeted therapy and single cell biology. however, the existing technologies are either of insufficient resolution, or unable to provide precise information on the localization of individual proteins. here, we report a new method that combines the use of quantum dot labeling, super-resolution microscopy (structured illumination microscopy, sim) and software modeling. in this proof-of-principle study, we assessed the biological effects of bestatin on individual cells from different aml cell lines expressing cd13 proteins, a potential target for tumor targeted therapy. using the proposed method, we found that the different aml cell lines exhibit different cd13 expression densities, ranging from 0.1 to 1.3 molecules per mu m(2) cell surface, respectively. importantly, bestatin treatment assays shows that its effects on cell growth inhibition, apoptosis and cell cycle change are directly proportional to the density of cd13 on the cell surface of these cell lines. the results suggest that the proposed method advances the quantitative analysis of single cell surface proteins, and that the quantitative profiling information of the target protein on single cells has potential value in targeted drug susceptibility assessment. |
| WOS关键词 | QUANTUM DOTS ; HETEROGENEITY ; CYTOMETRY |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| WOS类目 | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied |
| 语种 | 英语 |
| WOS记录号 | WOS:000459910900022 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373182 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Natl Lab Biomacromol, CAS Ctr Excellence Biomacromol, Inst Biophys, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Henan Univ, Coll Basic Med Sci, Kaifeng 475014, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xi, Yan,Wang, Dianbing,Wang, Tingting,et al. Quantitative profiling of cd13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment[J]. Nanoscale,2019,11(4):1737-1744. |
| APA | Xi, Yan,Wang, Dianbing,Wang, Tingting,Huang, Lin,&Zhang, Xian-En.(2019).Quantitative profiling of cd13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment.Nanoscale,11(4),1737-1744. |
| MLA | Xi, Yan,et al."Quantitative profiling of cd13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment".Nanoscale 11.4(2019):1737-1744. |
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来源：武汉病毒研究所
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