Phage reduce stability for regaining infectivity during antagonistic coevolution with host bacterium
文献类型：期刊论文
| 作者 | Yuan, Yihui1,2; Peng, Qin3; Zhang, Shaowen1; Liu, Tingting1; Yang, Shuo1; Yu, Qiuhan1; Wu, Yan2; Gao, Meiying2 |
| 刊名 | Viruses-basel
 |
| 出版日期 | 2019-02-01 |
| 卷号 | 11期号:2页码:14 |
| 关键词 | Bacteriophage Coevolution Synergism mutation Baseplate Flagellum |
| ISSN号 | 1999-4915 |
| DOI | 10.3390/v11020118 |
| 通讯作者 | Gao, meiying(mygao@wh.iov.cn) |
| 英文摘要 | The coevolution between phage and host bacterium is an important force that drives the evolution of the microbial community, yet the coevolution mechanisms have still not been well analyzed. here, by analyzing the interaction between a bacillus phage vb_bths_bmbphi and its host bacterium, the coevolution mechanisms of the first-generation phage-resistant bacterial mutants and regained-infectivity phage mutants were studied. the phage-resistant bacterial mutants showed several conserved mutations as a potential reason for acquiring phage resistance, including the mutation in flagellum synthesis protein flha and cell wall polysaccharide synthesis protein dltc. all the phage-resistant bacterial mutants showed a deleted first transmembrane domain of the flagellum synthesis protein flha. meanwhile, the regain-infectivity phage mutants all contained mutations in three baseplate-associated phage tail proteins by one nucleotide, respectively. a polymorphism analysis of the three mutant nucleotides in the wild-type phage revealed that the mutations existed before the interaction of the phage and the bacterium, while the wild-type phage could not infect the phage-resistant bacterial mutants, which might be because the synchronized mutations of the three nucleotides were essential for regaining infectivity. this study for the first time revealed that the synergism mutation of three phage baseplate-associated proteins were essential for the phages' regained infectivity. although the phage mutants regained infectivity, their storage stability was decreased and the infectivity against the phage-resistant bacterial mutants was reduced, suggesting the phage realized the continuation of the species by way of dying to survive. |
| WOS关键词 | BACILLUS-ANTHRACIS ; EVOLUTIONARY DYNAMICS ; GENOMIC ANALYSIS ; BACTERIOPHAGE ; RESISTANCE ; PATHOGEN ; THURINGIENSIS ; PROTEINS ; CEREUS ; J-1 |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000460803200028 |
| 出版者 | MDPI |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373184 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gao, Meiying |
| 作者单位 | 1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 3.Hainan Normal Univ, Coll Life Sci, Minist Educ, Key Lab Ecol Trop Isl, Haikou 571158, Hainan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Yihui,Peng, Qin,Zhang, Shaowen,et al. Phage reduce stability for regaining infectivity during antagonistic coevolution with host bacterium[J]. Viruses-basel,2019,11(2):14. |
| APA | Yuan, Yihui.,Peng, Qin.,Zhang, Shaowen.,Liu, Tingting.,Yang, Shuo.,...&Gao, Meiying.(2019).Phage reduce stability for regaining infectivity during antagonistic coevolution with host bacterium.Viruses-basel,11(2),14. |
| MLA | Yuan, Yihui,et al."Phage reduce stability for regaining infectivity during antagonistic coevolution with host bacterium".Viruses-basel 11.2(2019):14. |
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来源：武汉病毒研究所
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