Prion dimer is heterogenous and is modulated by multiple negative and positive motifs
文献类型：期刊论文
| 作者 | Gao, Zhenxing1,2; Shi, Jing3; Cai, Lili1; Luo, Minhua1; Wong, Boon-Seng4; Dong, Xiaoping5; Sy, Man-Sun6; Li, Chaoyang1,2 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2019-02-05 |
| 卷号 | 509期号:2页码:570-576 |
| 关键词 | Prion protein Disulfide bond Fatal familial insomnia Prp dimer Prp aggregation |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2018.12.113 |
| 通讯作者 | Li, chaoyang(cyli@wh.iov.cn) |
| 英文摘要 | The conversion of the normal prion protein (prp) into a scrapie priori (prpsc) is incompletely understood. theoretically, the smallest prp aggregate is a dimer. human prp contains two cysteines at positions 179 (c179) and 214 (c214) enabling disulfide bonding. here, we report that our recombinant human prp (r-hprp) preparations contain 0.2-0.8% dimer, which is linked by either one or two disulfide bonds, connected by c179-c179, c214-c214, or c179-c214. furthermore, dimerization is regulated by multiple motifs. while residues 36-42 inhibit, residues 90-125, and 195-212 promote dimerization. mutating individual residue between 36 and 42 enhances dimerization whereas mutating the positively charged residues within 95-115, or the negatively charged residues within 195-212 prevent dimerization. although deletion of the entire octapeptide-repeat (5or) region prevents dimerization, mutating the histidines within the 5or enhances dimerization. in addition, we found that two out of three brain lysates from patients with inherited prion disease had more prp dimers than controls. thus, prp dimerlzation may contribute to prion diseases. (c) 2018 elsevier inc. all rights reserved. |
| WOS关键词 | CONFORMATIONAL-CHANGES ; PROTEASE RESISTANCE ; PRP ; AGGREGATION ; RECOMBINANT ; CONVERSION ; MECHANISM ; PROTEINS ; BINDING ; DOMAIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000458094800037 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373190 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Li, Chaoyang |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, 44 Xiao Hong Shan Zhong Qu, Wuhan 430071, Hubei, Peoples R China 2.Guangzhou Med Univ, Affiliated Canc Hosp & Inst, 78 Heng Zhi Gang Rd, Guangzhou 510095, Guangdong, Peoples R China 3.Xiangyang Ctr Dis Control & Prevent, 172 Tan Xi Rd, Xiangyang, Hubei, Peoples R China 4.Singapore Inst Technol, Hlth & Social Sci Cluster, Singapore, Singapore 5.Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, State Key Lab Infect Dis Prevent & Control, Natl Inst Viral Dis Control & Prevent,Chinese Ctr, Chang Bai Rd 155, Beijing 102206, Peoples R China 6.Case Western Reserve Univ, Sch Med, Dept Pathol, 2103 Cornell Rd, Cleveland, OH 44106 USA |
| 推荐引用方式 GB/T 7714 | Gao, Zhenxing,Shi, Jing,Cai, Lili,et al. Prion dimer is heterogenous and is modulated by multiple negative and positive motifs[J]. Biochemical and biophysical research communications,2019,509(2):570-576. |
| APA | Gao, Zhenxing.,Shi, Jing.,Cai, Lili.,Luo, Minhua.,Wong, Boon-Seng.,...&Li, Chaoyang.(2019).Prion dimer is heterogenous and is modulated by multiple negative and positive motifs.Biochemical and biophysical research communications,509(2),570-576. |
| MLA | Gao, Zhenxing,et al."Prion dimer is heterogenous and is modulated by multiple negative and positive motifs".Biochemical and biophysical research communications 509.2(2019):570-576. |
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来源：武汉病毒研究所
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