Crystal structure of bacillus thuringiensis cry7ca1 toxin active against locusta migratoria manilensis
文献类型：期刊论文
| 作者 | Jing, Xuping1,2; Yuan, Yihui1; Wu, Yan1; Wu, Dandan1; Gong, Peng3; Gao, Meiying1 |
| 刊名 | Protein science
 |
| 出版日期 | 2019-03-01 |
| 卷号 | 28期号:3页码:609-619 |
| 关键词 | Bacillus thuringiensis Cry7ca1 toxin Crystal structure Locusta migratoria manilensis |
| ISSN号 | 0961-8368 |
| DOI | 10.1002/pro.3561 |
| 通讯作者 | Gong, peng(gongpeng@wh.iov.cn) ; Gao, meiying(mygao@wh.iov.cn) |
| 英文摘要 | Insecticidal crystal (cry) proteins produced by bacillus thuringiensis (bt) are widely used as environmentally friendly insecticides. as the only known cry protein with insecticidal activity against locusta migratoria manilensis, a locust subspecies that causes extensive destruction of crops, the cry7ca1 protein from bt strain bth-13 identified in our previous study is of particular interest to locust prevention and control. however, the three-dimensional structure of cry7ca1 toxin (the active form of the cry7ca1 protein) and the mechanisms of the cry7ca1 insecticidal specificity remain largely elusive. here, we report a 2.3 angstrom crystal structure of the cry7ca1 toxin and carry out a systematic comparison of all available cry toxins structures. a cluster of six loops in cry toxin domain ii, named apex here, are the most variable structural elements and were documented to contribute in insecticidal specificity. the cry7ca1 toxin apex loops are different from those of other cry toxins in length, conformation, and sequence. electrostatic potential analysis further revealed that cry7ca1 is the only structure-available cry toxin that does not have a high contrast of surface electrostatic potentials in the apex. we further suggest that the l1/l2 loops in the center of the cry7ca1 apex may be worthy of attention in future efforts to unravel the cry7ca1 insecticidal specificity as they exhibit unique features not found in the corresponding regions of other cry toxins. our work highlights the uniqueness of the apex in the cry7ca1 toxin and may assist exploration of the insecticidal mechanism of the cry7ca1 against locusta migratoria manilensis. |
| WOS关键词 | DELTA-ENDOTOXIN ; CRY1AB TOXIN ; BINDING ; CRYSTALLOGRAPHY ; SOFTWARE ; PROTEINS ; CRY4BA ; SYSTEM |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000458407700014 |
| 出版者 | WILEY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373191 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gong, Peng; Gao, Meiying |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jing, Xuping,Yuan, Yihui,Wu, Yan,et al. Crystal structure of bacillus thuringiensis cry7ca1 toxin active against locusta migratoria manilensis[J]. Protein science,2019,28(3):609-619. |
| APA | Jing, Xuping,Yuan, Yihui,Wu, Yan,Wu, Dandan,Gong, Peng,&Gao, Meiying.(2019).Crystal structure of bacillus thuringiensis cry7ca1 toxin active against locusta migratoria manilensis.Protein science,28(3),609-619. |
| MLA | Jing, Xuping,et al."Crystal structure of bacillus thuringiensis cry7ca1 toxin active against locusta migratoria manilensis".Protein science 28.3(2019):609-619. |
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来源：武汉病毒研究所
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