An insight into dna binding properties of newly designed cationic delta,delta '-diazacarbazoles: spectroscopy, afm imaging and living cells staining studies
文献类型：期刊论文
| 作者 | Jia, Tao1; Chen, Zhi-Hang3; Guo, Peng1; Yu, Junping2 |
| 刊名 | Spectrochimica acta part a-molecular and biomolecular spectroscopy
 |
| 出版日期 | 2019-03-15 |
| 卷号 | 211页码:260-271 |
| ISSN号 | 1386-1425 |
| 关键词 | Cationic delta,delta-diazacarbazole Dna binder Cd Uv-vis Fluorescence Afm |
| DOI | 10.1016/j.saa.2018.12.013 |
| 通讯作者 | Yu, junping(yujp@wh.iov.cn) |
| 英文摘要 | Two cationic delta,delta'-diazacarbazoles, 1-methyl-5h-pyrrolo[3,2-b:4,5-b']dipyridinium iodide (mpdpi) and 1,5-dimethyl-5h-pyrrolo[3,2-b:4,5-b']dipyridinium iodide (dpdpi), were devised and synthesized. through characterizations of the interactions between dna and the two delta,delta'-diazacarbazoles by various spectroscopy means, the strong interactions between the two compounds and double-strand dna have been observed and the interaction types and mechanisms were explored. uv-vis and fluorescent data have shown the big changes of dna in the presence of either of the two compounds, demonstrating that both of the delta,delta'-diazacarbazoles can bind to dna tightly, and high ionic strength decreased the intercalative interactions. the uv-vis and fluorescence of dsdna in the presence of dpdpi showed more profound changes than those in the presence of mpdpi, due to -ch3 (in the structure of dpdpi) taking place of h (in the structure of mpdpi) at the position of 5-nh. and the circular dichroism (cd) spectra of ct-dna and atomic force microscopy (afm) results indicated more compacted conformation of dna in the presence of dpdpi than mpdpi, implying that dpdpi has a more significant effect on dna conformations than mpdpi. most interestingly, fluorescence enhancement of cationic delta,delta'-diazacarbazoles occurred in the presence of dna. with ionic strength increasing, the intercalative interactions between delta,delta'-diazacarbazoles and dna were weakened, but delta,delta'-diazacarbazoles-dna complexes showed enhanced fluorescence, which indicated that there are other interactions present at high ionic strength. furthermore, laser confocal fluorescence microscopy results proved that dpdpi was membrane-permeable and stained living cells. (c) 2018 elsevier b.v. all rights reserved. |
| WOS关键词 | BETA-CYCLODEXTRIN ; ETHIDIUM-BROMIDE ; MINOR-GROOVE ; COMPLEXES ; MODES ; ACID |
| WOS研究方向 | Spectroscopy |
| WOS类目 | Spectroscopy |
| 语种 | 英语 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000457506200033 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373192 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yu, Junping |
| 作者单位 | 1.Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 3.Johns Hopkins Univ, Sch Med, Dept Radiol, Baltimore, MD 21205 USA |
| 推荐引用方式 GB/T 7714 | Jia, Tao,Chen, Zhi-Hang,Guo, Peng,et al. An insight into dna binding properties of newly designed cationic delta,delta '-diazacarbazoles: spectroscopy, afm imaging and living cells staining studies[J]. Spectrochimica acta part a-molecular and biomolecular spectroscopy,2019,211:260-271. |
| APA | Jia, Tao,Chen, Zhi-Hang,Guo, Peng,&Yu, Junping.(2019).An insight into dna binding properties of newly designed cationic delta,delta '-diazacarbazoles: spectroscopy, afm imaging and living cells staining studies.Spectrochimica acta part a-molecular and biomolecular spectroscopy,211,260-271. |
| MLA | Jia, Tao,et al."An insight into dna binding properties of newly designed cationic delta,delta '-diazacarbazoles: spectroscopy, afm imaging and living cells staining studies".Spectrochimica acta part a-molecular and biomolecular spectroscopy 211(2019):260-271. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。