Real-time dissection of dynamic uncoating of individual influenza viruses
文献类型：期刊论文
| 作者 | Qin, Chong1,2; Li, Wei1; Li, Qin1; Yin, Wen1,2; Zhang, Xiaowei1; Zhang, Zhiping1; Zhang, Xian-En3; Cui, Zongqiang1 |
| 刊名 | Proceedings of the national academy of sciences of the united states of america
 |
| 出版日期 | 2019-02-12 |
| 卷号 | 116期号:7页码:2577-2582 |
| 关键词 | Influenza virus Single-particle tracking Quantum dots Uncoating Vrnp dynamics |
| ISSN号 | 0027-8424 |
| DOI | 10.1073/pnas.1812632116 |
| 通讯作者 | Cui, zongqiang(czq@wh.iov.cn) |
| 英文摘要 | Uncoating is an obligatory step in the virus life cycle that serves as an antiviral target. unfortunately, it is challenging to study viral uncoating due to methodology limitations for detecting this transient and dynamic event. the uncoating of influenza a virus (iav), which contains an unusual genome of eight segmented rnas, is particularly poorly understood. here, by encapsulating quantum dot (qd)-conjugated viral ribonucleoprotein complexes (vrnps) within infectious iav virions and applying single-particle imaging, we tracked the uncoating process of individual iav virions. approximately 30% of iav particles were found to undergo uncoating through fusion with late endosomes in the "around-nucleus" region at 30 to 90 minutes postinfection. inhibition of viral m2 proton channels and cellular endosome acidification prevented iav uncoating. iav vrnps are released separately into the cytosol after virus uncoating. then, individual vrnps undergo a three-stage movement to the cell nucleus and display two diffusion patterns when inside the nucleus. these findings reveal iav uncoating and vrnp trafficking mechanisms, filling a critical gap in knowledge about influenza viral infection. |
| WOS关键词 | SINGLE-PARTICLE TRACKING ; NUCLEAR IMPORT ; INFECTION ; TRANSPORT ; MACHINERY ; RNA |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000458365600033 |
| 出版者 | NATL ACAD SCIENCES |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373194 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Cui, Zongqiang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Chong,Li, Wei,Li, Qin,et al. Real-time dissection of dynamic uncoating of individual influenza viruses[J]. Proceedings of the national academy of sciences of the united states of america,2019,116(7):2577-2582. |
| APA | Qin, Chong.,Li, Wei.,Li, Qin.,Yin, Wen.,Zhang, Xiaowei.,...&Cui, Zongqiang.(2019).Real-time dissection of dynamic uncoating of individual influenza viruses.Proceedings of the national academy of sciences of the united states of america,116(7),2577-2582. |
| MLA | Qin, Chong,et al."Real-time dissection of dynamic uncoating of individual influenza viruses".Proceedings of the national academy of sciences of the united states of america 116.7(2019):2577-2582. |
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来源：武汉病毒研究所
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