Identification and genomic sequence analysis of a new spodoptera exigua multiple nucleopolyhedrovirus, semnpv-qd, isolated from qingdao, china
文献类型：期刊论文
| 作者 | Chen, Yingjian1; Qi, Benxiang1; Zheng, Guiling1; Zhang, Yuan1; Deng, Fei2; Wan, Fanghao1,3; Li, Changyou1 |
| 刊名 | Journal of invertebrate pathology
 |
| 出版日期 | 2019 |
| 卷号 | 160页码:8-17 |
| 关键词 | Baculovirus Nucleopolyhedrovirus Spodoptera exigua Genome sequence Phylogenetic tree |
| ISSN号 | 0022-2011 |
| DOI | 10.1016/j.jip.2018.11.006 |
| 通讯作者 | Li, changyou(cyli@qau.edu.cn) |
| 英文摘要 | A new beet armyworm (spodoptera exigua) multiple nucleopolyhedrovirus semnpv-qd was isolated from dead larvae in the field in qingdao, shandong, china. the virus has a polyhedron size of 1.39 +/- 0.28 mu m, and typical virions contain one to seven nucleocapsids per envelope. semnpv-qd only infects the larvae of s. exigua; it does not infect larvae of s. litura, agrotis ipsilon, a. segetum, bombyx mori, hyphantria cunea, or stilpnotia salicis, and thus, has higher host specificity. semnpv-qd has a circular double-stranded dna genome of 128,525 bp with a gc content of 37.41% and 127 putative open reading frames (orf), each of which encodes more than 50 amino acids. these were identified and annotated in the semnpv-qd genome, accounting for 87.53% of the whole genome. phylogenetic analysis of 38 core genes of the baculovirus confirmed that semnpv-qd is a group ii alphabaculovirus and is most closely related to semnpv american isolate (semnpv-us1), s. litura nucleopolyhedrovirus ii (spiinpv-ii), s. frugiperda multiple nucleopolyhedrovirus (sfmnpv), a. segetum nucleopolyhedrovirus (agsenpv), a. segetum nucleopolyhedrovirus b (agsenpv-b) and a. ipsilon multiple nucleopolyhedrovirus (agipnpv). the pairwise distance of the nucleotide sequences of lef-8, lef-9, polh and concatenated lef-8/lef-9/polh fragments between semnpv-qd and several sister viruses mentioned above were all above 0.05 substitutions/site. semnpv-qd has 123 orfs similar to those of semnpv-us1, and the genomic similarity was 45.8%. compared to semnpv-us1, semnpv-qd has four additional orfs such as two baculovirus repeat orf (bro) genes, bro-1, bro-2, orf39 and orf95, but lacks 17 orfs that have no effect on viral transcription and replication. the above results indicate that semnpv-qd is a new species of s. exigua multiple nucleopolyhedrovirus. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; BEET ARMYWORM ; BACULOVIRUSES ; LEPIDOPTERA ; BRO ; METHOXYFENOZIDE ; CLASSIFICATION ; NOMENCLATURE ; POPULATIONS ; RESISTANCE |
| WOS研究方向 | Zoology |
| WOS类目 | Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000455857600002 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373201 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Li, Changyou |
| 作者单位 | 1.Qingdao Agr Univ, Coll Plant Hlth & Med, Key Lab Integrated Crop Pest Management Shandong, Qingdao 266109, Shandong, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yingjian,Qi, Benxiang,Zheng, Guiling,et al. Identification and genomic sequence analysis of a new spodoptera exigua multiple nucleopolyhedrovirus, semnpv-qd, isolated from qingdao, china[J]. Journal of invertebrate pathology,2019,160:8-17. |
| APA | Chen, Yingjian.,Qi, Benxiang.,Zheng, Guiling.,Zhang, Yuan.,Deng, Fei.,...&Li, Changyou.(2019).Identification and genomic sequence analysis of a new spodoptera exigua multiple nucleopolyhedrovirus, semnpv-qd, isolated from qingdao, china.Journal of invertebrate pathology,160,8-17. |
| MLA | Chen, Yingjian,et al."Identification and genomic sequence analysis of a new spodoptera exigua multiple nucleopolyhedrovirus, semnpv-qd, isolated from qingdao, china".Journal of invertebrate pathology 160(2019):8-17. |
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来源：武汉病毒研究所
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