Genetic evidence of middle east respiratory syndrome coronavirus (mers-cov) and widespread seroprevalence among camels in kenya
文献类型：期刊论文
| 作者 | Ommeh, Sheila1; Zhang, Wei2; Zohaib, Ali2; Chen, Jing2; Zhang, Huajun2; Hu, Ben2; Ge, Xing-Yi3; Yang, Xing-Lou2; Masika, Moses4; Obanda, Vincent5 |
| 刊名 | Virologica sinica
 |
| 出版日期 | 2018-12-01 |
| 卷号 | 33期号:6页码:484-492 |
| ISSN号 | 1674-0769 |
| 关键词 | Middle east respiratory syndrome coronavirus (mers-cov) One-health Public health Zoonosis Kenya |
| DOI | 10.1007/s12250-018-0076-4 |
| 通讯作者 | Agwanda, bernard(bagwanda@museums.or.ke) ; Shi, zheng-li(zlshi@wh.iov.cn) |
| 英文摘要 | We describe the first genome isolation of middle east respiratory syndrome coronavirus (mers-cov) in kenya. this fatal zoonotic pathogen was first described in the kingdom of saudi arabia in 2012. epidemiological and molecular evidence revealed zoonotic transmission from camels to humans and between humans. currently, mers-cov is classified by the who as having high pandemic potential requiring greater surveillance. previous studies of mers-cov in kenya mainly focused on site-specific and archived camel and human serum samples for antibodies. we conducted active nationwide cross-sectional surveillance of camels and humans in kenya, targeting both nasal swabs and plasma samples from 1,163 camels and 486 humans collected from january 2016 to june 2018. a total of 792 camel plasma samples were positive by elisa. seroprevalence increased with age, and the highest prevalence was observed in adult camels (82.37%, 95% confidence interval (ci) 79.50-84.91). more female camels were significantly seropositive (74.28%, 95% ci 71.14-77.19) than male camels (p<0.001) (53.74%, 95% ci 48.48-58.90). only 11 camel nasal swabs were positive for mers-cov by reverse transcription-quantitative pcr. phylogenetic analysis of whole genome sequences showed that kenyan mers-cov clustered within sub-clade c2, which is associated with the african clade, but did not contain signature deletions of orf4b in african viruses. none of the human plasma screened contained neutralizing antibodies against mers-cov. this study confirms the geographically widespread occurrence of mers-cov in kenyan camels. further one-health surveillance approaches in camels, wildlife, and human populations are needed. |
| WOS关键词 | DROMEDARY CAMELS ; ANTIBODIES ; AFRICA |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000455958600004 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373204 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Agwanda, Bernard; Shi, Zheng-Li |
| 作者单位 | 1.Jomo Kenyatta Univ Agr & Technol, Inst Biotechnol Res, Nairobi 6200000200, Kenya 2.Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 3.Hunan Univ, Coll Biol, Changsha 410006, Hunan, Peoples R China 4.Univ Nairobi, Dept Med Microbiol, Nairobi 3019700100, Kenya 5.Kenya Wildlife Serv, Vet Serv Dept, Nairobi 4024100100, Kenya 6.Kenya Agr & Livestock Res Org, Vet Res Inst, Nairobi 5781100200, Kenya 7.Duke NUS Med Sch, Vet Serv Programme Emerging Infect Dis, Singapore 169857, Singapore 8.Minist Agr Livestock Fisheries & Irrigat, State Dept Livestock, Directorate Vet Serv, Nairobi 3418800100, Kenya 9.Kenya Camel Assoc, Nairobi 3009500100, Kenya 10.Natl Museums Kenya, Dept Zool, Nairobi 4065800100, Kenya |
| 推荐引用方式 GB/T 7714 | Ommeh, Sheila,Zhang, Wei,Zohaib, Ali,et al. Genetic evidence of middle east respiratory syndrome coronavirus (mers-cov) and widespread seroprevalence among camels in kenya[J]. Virologica sinica,2018,33(6):484-492. |
| APA | Ommeh, Sheila.,Zhang, Wei.,Zohaib, Ali.,Chen, Jing.,Zhang, Huajun.,...&Shi, Zheng-Li.(2018).Genetic evidence of middle east respiratory syndrome coronavirus (mers-cov) and widespread seroprevalence among camels in kenya.Virologica sinica,33(6),484-492. |
| MLA | Ommeh, Sheila,et al."Genetic evidence of middle east respiratory syndrome coronavirus (mers-cov) and widespread seroprevalence among camels in kenya".Virologica sinica 33.6(2018):484-492. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。