Rna binding protein 24 regulates the translation and replication of hepatitis c virus
文献类型：期刊论文
| 作者 | Cao, Huang1,2; Zhao, Kaitao1,2; Yao, Yongxuan1,2; Guo, Jing1,2; Gao, Xiaoxiao1,2; Yang, Qi1,2; Guo, Min1,2; Zhu, Wandi1; Wang, Yun1; Wu, Chunchen1 |
| 刊名 | Protein & cell
 |
| 出版日期 | 2018-11-01 |
| 卷号 | 9期号:11页码:930-944 |
| ISSN号 | 1674-800X |
| 关键词 | Rna binding protein Rbm24 Hepatitis c virus Translation Replication |
| DOI | 10.1007/s13238-018-0507-x |
| 通讯作者 | Chen, xinwen(chenxw@wh.iov.cn) ; Pei, rongjuan(rongjuan_pei@wh.iov.cn) |
| 英文摘要 | The secondary structures of hepatitis c virus (hcv) rna and the cellular proteins that bind to them are important for modulating both translation and rna replication. however, the sets of rna-binding proteins involved in the regulation of hcv translation, replication and encapsidation remain unknown. here, we identified rna binding motif protein 24 (rbm24) as a host factor participated in hcv translation and replication. knockdown of rbm24 reduced hcv propagation in huh7.5.1 cells. an enhanced translation and delayed rna synthesis during the early phase of infection was observed in rbm24 silencing cells. however, both overexpression of rbm24 and recombinant human rbm24 protein suppressed hcv ires-mediated translation. further analysis revealed that the assembly of the 80s ribosome on the hcv ires was interrupted by rbm24 protein through binding to the 5-utr. rbm24 could also interact with hcv core and enhance the interaction of core and 5-utr, which suppresses the expression of hcv. moreover, rbm24 enhanced the interaction between the 5- and 3-utrs in the hcv genome, which probably explained its requirement in hcv genome replication. therefore, rbm24 is a novel host factor involved in hcv replication and may function at the switch from translation to replication. |
| WOS关键词 | RIBOSOME ENTRY SITE ; 5' UNTRANSLATED REGION ; CORE PROTEIN ; DEPENDENT TRANSLATION ; SECONDARY STRUCTURE ; 80S RIBOSOME ; DOMAIN-II ; STEM-LOOP ; IN-VITRO ; 3' END |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| 出版者 | SPRINGEROPEN |
| WOS记录号 | WOS:000452190200003 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373206 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Xinwen; Pei, Rongjuan |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Duisburg Essen, Univ Hosp Essen, Dept Infect Dis, Gebaude V15, D-45117 Essen, Germany |
| 推荐引用方式 GB/T 7714 | Cao, Huang,Zhao, Kaitao,Yao, Yongxuan,et al. Rna binding protein 24 regulates the translation and replication of hepatitis c virus[J]. Protein & cell,2018,9(11):930-944. |
| APA | Cao, Huang.,Zhao, Kaitao.,Yao, Yongxuan.,Guo, Jing.,Gao, Xiaoxiao.,...&Pei, Rongjuan.(2018).Rna binding protein 24 regulates the translation and replication of hepatitis c virus.Protein & cell,9(11),930-944. |
| MLA | Cao, Huang,et al."Rna binding protein 24 regulates the translation and replication of hepatitis c virus".Protein & cell 9.11(2018):930-944. |
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来源：武汉病毒研究所
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