Self-assembly of antigen proteins into nanowires greatly enhances the binding affinity for high-efficiency target capture
文献类型：期刊论文
| 作者 | Men, Dong1; Zhou, Juan1; Li, Wei3; Wei, Cui-Hua1; Chen, Yuan-Yuan4; Zhou, Kun1; Zheng, Ying1; Xu, Ke5; Zhang, Zhi-Ping1; Zhang, Xian-En2 |
| 刊名 | Acs applied materials & interfaces
 |
| 出版日期 | 2018-12-05 |
| 卷号 | 10期号:48页码:41019-41025 |
| 关键词 | Self-assembly Protein nanowire Polyvalent interaction Binding affinity Sensitive immunoassay |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.8b12511 |
| 通讯作者 | Men, dong(d.men@wh.iov.cn) ; Zhang, xian-en(zhangxe@ibp.ac.cn) |
| 英文摘要 | High-efficiency target capture is an essential prerequisite for sensitive immunoassays. however, the current available immunoassay approaches are subject to deficient binding affinities between predator-prey molecules that greatly restrict the target capture efficiency and immunoassay sensitivity. herein, we present a new strategy through the self-assembly of antigen proteins into nanowires to enhance the binding affinity between an antigen and antibody. through the genetic fusion of antigen proteins (e.g., hiv p24) with the yeast amyloid protein sup35 self-assembly domain, specific antigen nanowires (ag nanowires) were constructed and demonstrated a remarkable enhancement in binding affinity compared with that of the monomeric antigen molecule. the ag nanowires were further combined with magnetic beads to form a 3d magnetic probe based on a seed-induced self-assembly strategy. taking advantage of both the strong binding affinity and the rapid magnetic separation and enrichment capacity, the specific 3d magnetic probe achieved a 100-fold improvement in detection sensitivity within a significantly shorter period of 20 min over that of the conventional enzyme-linked immunosorbent assay method. |
| WOS关键词 | MULTIVALENT ; BIOSENSORS ; LIGANDS ; DESIGN ; ASSAYS |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000452694100009 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373208 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Men, Dong; Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China 3.Hubei Univ, Coll Life Sci, Wuhan 430062, Hubei, Peoples R China 4.Huazhong Agr Univ, Wuhan 430070, Hubei, Peoples R China 5.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Pasteur Shanghai, CAS Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Men, Dong,Zhou, Juan,Li, Wei,et al. Self-assembly of antigen proteins into nanowires greatly enhances the binding affinity for high-efficiency target capture[J]. Acs applied materials & interfaces,2018,10(48):41019-41025. |
| APA | Men, Dong.,Zhou, Juan.,Li, Wei.,Wei, Cui-Hua.,Chen, Yuan-Yuan.,...&Zhang, Xian-En.(2018).Self-assembly of antigen proteins into nanowires greatly enhances the binding affinity for high-efficiency target capture.Acs applied materials & interfaces,10(48),41019-41025. |
| MLA | Men, Dong,et al."Self-assembly of antigen proteins into nanowires greatly enhances the binding affinity for high-efficiency target capture".Acs applied materials & interfaces 10.48(2018):41019-41025. |
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来源：武汉病毒研究所
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