Global profiling of pkng interactions using a human proteome microarray reveals novel connections with cypa
文献类型：期刊论文
| 作者 | Wu, Fan-Lin1,2,3; Liu, Yin1,2; Zhang, Hai-Nan1,2; Jiang, He-Wei1,2; Cheng, Li1,2; Guo, Shu-Juan1,2; Deng, Jiao-Yu5; Bi, Li-Jun6,7,8,9,10; Zhang, Xian-En6,7; Gao, Hua-Fang4 |
| 刊名 | Proteomics
 |
| 出版日期 | 2018-12-01 |
| 卷号 | 18期号:23页码:13 |
| ISSN号 | 1615-9853 |
| 关键词 | Pkng Huprot array Cypa Nf-kappa b Erk1/2 |
| DOI | 10.1002/pmic.201800265 |
| 通讯作者 | Gao, hua-fang(gaohuafang@nrifp.org.cn) ; Tao, sheng-ce(taosc@sjtu.edu.cn) |
| 英文摘要 | Mycobacterium tuberculosis (mtb) serine/threonine kinase pkng plays an important role in the mtb-host interaction by facilitating the survival of mtb in macrophages. however, the human proteins with which the pkng interacts, and the underlying molecular mechanisms are still largely unknown. in this study, a huprot array is been applied to globally identify the host proteins to which pkng binds. in this way, 125 interactors are discovered, including a cyclophilin protein, cypa. this interaction between pkng and cypa is validated both in vitro and in vivo, and functional studies show that pkng significantly reduces the protein levels of cypa through phosphorylation, which consequently inhibit the inflammatory response through downregulation of nf-kappa b and erk1/2 pathways. phenotypically, overexpression of pkng reduces cytokine levels and promotes the survival of mycobacterium smegmatis (msm) in macrophages. overall, it is expected that the pkng interactors identified in this study will serve as a useful resource for further systematic studies of the roles that pkng plays in the mtb-host interactions. |
| WOS关键词 | MYCOBACTERIUM-TUBERCULOSIS ; SYSTEMATIC IDENTIFICATION ; KINASE PKNG ; PHOSPHORYLATION ; CHOLESTEROL ; CYCLOPHILIN ; PROTEINS ; SEQUENCE ; SURVIVAL ; PATHWAY |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000452425500008 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373213 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gao, Hua-Fang; Tao, Sheng-Ce |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Minist Educ, Shanghai 200240, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200240, Peoples R China 3.Ludong Univ, Sch Agr, Yantai 264025, Peoples R China 4.Natl Res Inst Hlth & Family Planning, Beijing 100081, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 6.Chinese Acad Sci, Inst Biophys, Natl Key Lab Biomacromol, Key Lab Noncoding RNA, Beijing 100101, Peoples R China 7.Chinese Acad Sci, Inst Biophys, Key Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China 8.Foshan Univ, Sch Stomatol & Med, Foshan 528000, Guangdong, Peoples R China 9.TB Healthcare Biotechnol Co Ltd, Foshan 528000, Guangdong, Peoples R China 10.Guangdong Prov Key Lab TB Syst Biol & Translat Me, Foshan 528000, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Fan-Lin,Liu, Yin,Zhang, Hai-Nan,et al. Global profiling of pkng interactions using a human proteome microarray reveals novel connections with cypa[J]. Proteomics,2018,18(23):13. |
| APA | Wu, Fan-Lin.,Liu, Yin.,Zhang, Hai-Nan.,Jiang, He-Wei.,Cheng, Li.,...&Tao, Sheng-Ce.(2018).Global profiling of pkng interactions using a human proteome microarray reveals novel connections with cypa.Proteomics,18(23),13. |
| MLA | Wu, Fan-Lin,et al."Global profiling of pkng interactions using a human proteome microarray reveals novel connections with cypa".Proteomics 18.23(2018):13. |
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来源：武汉病毒研究所
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