Three-fragment fluorescence complementation for imaging of ternary complexes under physiological conditions
文献类型：期刊论文
| 作者 | Chen, Minghai1,3; Li, Wei1; Zhang, Zhi-Ping1; Pan, Jingdi1,3; Sun, Yuhan1,3; Zhang, Xiaowei1; Zhang, Xian-En2; Cui, Zongqiang1 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2018-11-20 |
| 卷号 | 90期号:22页码:13299-13305 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b02661 |
| 通讯作者 | Cui, zongqiang(czq@wh.iov.cn) |
| 英文摘要 | Protein protein interactions (ppis) occur in a vast variety of cellular processes, and many processes are regulated by multiple protein interactions. identification of ppis is essential for the analysis of biological pathways and to further understand underlying molecular mechanisms. however, visualization and identification of multiprotein complexes, including ternary complexes in living cells under physiological conditions, remains challenging. in this work, we reported a three-fragment fluorescence complementation (tffc) by splitting the venus fluorescent protein for visualizing ternary complexes in living cells under physiological conditions. with this venus-based tffc system, we identified the multi-interaction of weak-affinity ternary complexes under physiological conditions. the tffc system was further applied to the analysis of multi-interactions during the hiv-1 integration process, revealing the important role of the barrier-to-autointegration factor protein in hiv-1 integration. this tffc system provides a useful tool for visualizing and identifying ternary complexes in living cells under physiological conditions. |
| WOS关键词 | LIVING CELLS ; RETROVIRAL DNA ; LEDGF/P75 ; PROTEIN ; BAF ; AUTOINTEGRATION ; VISUALIZATION ; INTEGRATION ; MICROSCOPY |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000451246100025 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373219 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Cui, Zongqiang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Minghai,Li, Wei,Zhang, Zhi-Ping,et al. Three-fragment fluorescence complementation for imaging of ternary complexes under physiological conditions[J]. Analytical chemistry,2018,90(22):13299-13305. |
| APA | Chen, Minghai.,Li, Wei.,Zhang, Zhi-Ping.,Pan, Jingdi.,Sun, Yuhan.,...&Cui, Zongqiang.(2018).Three-fragment fluorescence complementation for imaging of ternary complexes under physiological conditions.Analytical chemistry,90(22),13299-13305. |
| MLA | Chen, Minghai,et al."Three-fragment fluorescence complementation for imaging of ternary complexes under physiological conditions".Analytical chemistry 90.22(2018):13299-13305. |
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来源：武汉病毒研究所
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