Vb_lspm-01: a novel myovirus displaying pseudolysogeny in lysinibacillus sphaericus c3-41
文献类型：期刊论文
| 作者 | Geng, Peiling1,2; Wan, Xiaofu1,2; Cheng, Jiao1,2; Yuan, Zhiming1; Hu, Xiaomin1 |
| 刊名 | Applied microbiology and biotechnology
 |
| 出版日期 | 2018-12-01 |
| 卷号 | 102期号:24页码:10691-10702 |
| ISSN号 | 0175-7598 |
| 关键词 | Lysinibacillus sphaericus Myovirus Lysis Lysogeny Pseudolysogeny Vb_lspm-01 |
| DOI | 10.1007/s00253-018-9424-4 |
| 通讯作者 | Yuan, zhiming(yzm@wh.iov.cn) ; Hu, xiaomin(huxm@wh.iov.cn) |
| 英文摘要 | Lysinibacillus sphaericus has great application potential not only in the biocontrol of mosquitoes but also in the bioremediation of toxic metals. phages contribute to the genetic diversity and niche adaptation of bacteria, playing essential roles in their life cycle, but may also cause economic damage for industrially important bacteria through phage contamination during fermentation. in this study, the l. sphaericus phage vb_lspm-01, which belongs to the myoviridae family, was isolated and characterized. results showed that vb_lspm-01 could specifically infect most tested l. sphaericus isolates but was not active against isolates belonging to other species. furthermore, phage-born endolysin exhibited a broader antimicrobial spectrum than the host range of the phage. the vb_lspm-01 genome had no obvious similarity with that of its host, and ca. 22.6% of putative orfs could not get a match with the public databases. phylogenic analysis based on the putative terminase large subunit showed high similarity with the phages identified with pac-type headful packaging. the vb_lspm-01 encoding genes were only detected in a tiny percentage of l. sphaericus c3-41 individual cells in the wild population, whereas they showed much higher frequency in the resistant population grown within the plaques; however, the phage genes could not be stably inherited during host cell division. additionally, the vb_lspm-01 encoding genes were only detected in the host population during the logarithmic growth phase. the mitomycin c induction helped the propagation and lysogeny-lysis switch of vb_lspm-01. the study demonstrated that vb_lspm-01 can be present in a pseudolysogenic state in l. sphaericus c3-41 populations. |
| WOS关键词 | BACILLUS-SPHAERICUS ; GENOME SEQUENCE ; CULEX-QUINQUEFASCIATUS ; UNSTABLE LYSOGENY ; LARGE SUBUNIT ; COS-SITE ; BACTERIOPHAGE ; DNA ; CEREUS ; IDENTIFICATION |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000451523800027 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373220 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan, Zhiming; Hu, Xiaomin |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Geng, Peiling,Wan, Xiaofu,Cheng, Jiao,et al. Vb_lspm-01: a novel myovirus displaying pseudolysogeny in lysinibacillus sphaericus c3-41[J]. Applied microbiology and biotechnology,2018,102(24):10691-10702. |
| APA | Geng, Peiling,Wan, Xiaofu,Cheng, Jiao,Yuan, Zhiming,&Hu, Xiaomin.(2018).Vb_lspm-01: a novel myovirus displaying pseudolysogeny in lysinibacillus sphaericus c3-41.Applied microbiology and biotechnology,102(24),10691-10702. |
| MLA | Geng, Peiling,et al."Vb_lspm-01: a novel myovirus displaying pseudolysogeny in lysinibacillus sphaericus c3-41".Applied microbiology and biotechnology 102.24(2018):10691-10702. |
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来源：武汉病毒研究所
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