Autographa californica multiple nucleopolyhedrovirus enters host cells via clathrin-mediated endocytosis and direct fusion with the plasma membrane
文献类型：期刊论文
| 作者 | Qin, Fujun1,2; Xu, Congrui1,2; Lei, Chengfeng1; Hu, Jia1; Sun, Xiulian1 |
| 刊名 | Viruses-basel
 |
| 出版日期 | 2018-11-01 |
| 卷号 | 10期号:11页码:17 |
| 关键词 | Acmnpv Single-virus tracking Quantitative electron microscopy Clathrin-mediated endocytosis Direct fusion with the plasma membrane |
| ISSN号 | 1999-4915 |
| DOI | 10.3390/v10110632 |
| 通讯作者 | Sun, xiulian(sunxl@wh.iov.cn) |
| 英文摘要 | The cell entry mechanism of autographa californica multiple nucleopolyhedrovirus (acmnpv) is not fully understood. previous studies showed that acmnpv entered host cells primarily through clathrin-mediated endocytosis, and could efficiently infect cells via fusion with the plasma membrane after a low-ph trigger. however, whether acmnpv enters cells via these two pathways simultaneously, and the exact manner in which acmnpv particles are internalized into cells remains unclear. in this study, using single-virus tracking, we observed that acmnpv particles were first captured by pre-existing clathrin-coated pits (ccp), and were then delivered to early endosomes. population-based analysis of single-virus tracking and quantitative electron microscopy demonstrated that the majority of particles were captured by ccps and internalized via invagination. in contrast, a minority of virus particles were not delivered to ccps, and were internalized through direct fusion with the plasma membrane without invagination. quantitative electron microscopy also showed that, while inhibition of ccp assembly significantly impaired viral internalization, inhibition of endosomal acidification blocked virus particles out of vesicles. collectively, these findings demonstrated that approximately 90% of acmnpv particles entered cells through clathrin-mediated endocytosis and 10% entered via direct fusion with the plasma membrane. this study will lead toward a better understanding of acmnpv infection. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; MAMMALIAN-CELLS ; PROTEIN EXPRESSION ; QUANTUM DOTS ; BACULOVIRUS ; ENTRY ; INSECT ; MECHANISM ; INFECTION ; TRANSPORT |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000451657600055 |
| 出版者 | MDPI |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373222 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Sun, Xiulian |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Fujun,Xu, Congrui,Lei, Chengfeng,et al. Autographa californica multiple nucleopolyhedrovirus enters host cells via clathrin-mediated endocytosis and direct fusion with the plasma membrane[J]. Viruses-basel,2018,10(11):17. |
| APA | Qin, Fujun,Xu, Congrui,Lei, Chengfeng,Hu, Jia,&Sun, Xiulian.(2018).Autographa californica multiple nucleopolyhedrovirus enters host cells via clathrin-mediated endocytosis and direct fusion with the plasma membrane.Viruses-basel,10(11),17. |
| MLA | Qin, Fujun,et al."Autographa californica multiple nucleopolyhedrovirus enters host cells via clathrin-mediated endocytosis and direct fusion with the plasma membrane".Viruses-basel 10.11(2018):17. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。