Rbpa relaxes promoter selectivity of m-tuberculosis rna polymerase
文献类型：期刊论文
| 作者 | Perumal, Ayyappasamy Sudalaiyadum1,3; Vishwakarma, Rishi Kishore1,4; Hu, Yangbo2; Morichaud, Zakia1; Brodolin, Konstantin1 |
| 刊名 | Nucleic acids research
 |
| 出版日期 | 2018-11-02 |
| 卷号 | 46期号:19页码:10106-10118 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gky714 |
| 通讯作者 | Brodolin, konstantin(konstantin.brodolin@irim.cnrs.fr) |
| 英文摘要 | The transcriptional activator rbpa associates with mycobacterium tuberculosis rna polymerase (mtbrnap) during transcription initiation, and stimulates formation of the mtbrnap-promoter open complex (rpo). here, we explored the influence of promoter motifs on rbpa-mediated activation of mtbrnap containing the stress-response sigma(b) subunit. we show that both the 'extended -10' promoter motif (t(-17)g(-16)t(-15)g(-14)) and rbpa stabilized rpo and allowed promoter opening at suboptimal temperatures. furthermore, in the presence of the t(-17)g(-16)t(-15)g(-14) motif, rbpa was dispensable for rna synthesis initiation, while exerting a stabilization effect on rpo. on the other hand, rbpa compensated for the lack of sequence-specific interactions of domains 3 and 4 of sigma(b) with the extended -10 and the -35 motifs, respectively. mutations of the positively charged residues k73, k74 and r79 in rbpa basic linker (bl) had little effect on rpo formation, but affected mtbrnap capacity for de novo transcription initiation. we propose that rbpa stimulates transcription by strengthening the non-specific interaction of the sigma subunit with promoter dna upstream of the -10 element, and by indirectly optimizing mtbrnap interaction with initiation substrates. consequently, rbpa renders mtbrnap promiscuous in promoter selection, thus compensating for the weak conservation of the -35 motif in mycobacteria. |
| WOS关键词 | OPEN-COMPLEX-FORMATION ; SIGMA(70) SUBUNIT ; TEMPERATURE-DEPENDENCE ; BACILLUS-SUBTILIS ; STRUCTURAL BASIS ; TRANSCRIPTION ; PROTEIN ; REGION ; MOTIF ; RECOGNITION |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000450955800022 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373223 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Brodolin, Konstantin |
| 作者单位 | 1.Univ Montpellier, CNRS, IRIM, 1919 Route Mende, F-34293 Montpellier, France 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 3.McGill Univ, Dept Bioengn, Montreal, PQ H3A 0C3, Canada 4.Univ Montpellier, CNRS, INSERM, CBS, Montpellier, France |
| 推荐引用方式 GB/T 7714 | Perumal, Ayyappasamy Sudalaiyadum,Vishwakarma, Rishi Kishore,Hu, Yangbo,et al. Rbpa relaxes promoter selectivity of m-tuberculosis rna polymerase[J]. Nucleic acids research,2018,46(19):10106-10118. |
| APA | Perumal, Ayyappasamy Sudalaiyadum,Vishwakarma, Rishi Kishore,Hu, Yangbo,Morichaud, Zakia,&Brodolin, Konstantin.(2018).Rbpa relaxes promoter selectivity of m-tuberculosis rna polymerase.Nucleic acids research,46(19),10106-10118. |
| MLA | Perumal, Ayyappasamy Sudalaiyadum,et al."Rbpa relaxes promoter selectivity of m-tuberculosis rna polymerase".Nucleic acids research 46.19(2018):10106-10118. |
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来源：武汉病毒研究所
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