Exploring a phage-based real-time pcr assay for diagnosing acinetobacter baumannii bloodstream infections with high sensitivity
文献类型：期刊论文
| 作者 | Luo, Jun1,2,3,4; Jiang, Mengwei1; Xiong, Jin1; Li, Junhua1; Zhang, Xiaoxu1,3; Wei, Hongping1; Yu, Junping1 |
| 刊名 | Analytica chimica acta
 |
| 出版日期 | 2018-12-31 |
| 卷号 | 1044页码:147-153 |
| ISSN号 | 0003-2670 |
| 关键词 | Phage Qpcr Bloodstream infections Bacteria Detection |
| DOI | 10.1016/j.aca.2018.09.038 |
| 通讯作者 | Wei, hongping(hpwei@wh.iov.cn) ; Yu, junping(yujp@wh.iov.cn) |
| 英文摘要 | In the present study, we developed a phage-based real-time quantitative pcr (qpcr) methodology for sensitive diagnosis of bloodstream infection (bsi) caused by acinetobacter baumannii (a. baumannii). an isolated a. baumannii phage p53 was used for taqman qpcr through detecting phage replication in live a. baumannii cells in serum samples. at the phage concentration of 10(3) pfu/ml, the sensitive detection of a. baumannii (down to 10 cfu in 100 mu l serum) has been obtained within 4 h in spiked serum samples without bacteria isolation and dna extraction. subsequent testing of 22 simulated serum samples spiked by different strains has shown that the results from the phage-based taqman qpcr method have 100% agreement with the spiked concentrations of the bacteria. the assay built in this study, gathering all the advantages for detections of high rapidity, high sensitivity, good specificity, being able to detect only live bacteria not dead bacteria and no dna extraction or purifications, can be developed to detecting other bacterial pathogens in serum or other complicated samples through switching to other types of phages and realize the rapid and sensitive detection of bacteria in bsi, which would potentially be applied for fast diagnosis in sepsis clinically. (c) 2018 elsevier b.v. all rights reserved. |
| WOS关键词 | COMBINING PHAGOMAGNETIC SEPARATION ; SEVERE SEPSIS ; ACOUSTIC SEPARATION ; SEPTIC SHOCK ; BACTERIA ; PATHOGENS ; IDENTIFICATION ; THERAPY ; COLI |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000449814800013 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373224 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wei, Hongping; Yu, Junping |
| 作者单位 | 1.Chinese Acad Sci, Ctr Emerging Infect Dis, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.China Three Gorges Univ, Coll Clin Med Sci 1, Yichang, Peoples R China 4.Yichang Cent Peoples Hosp, Yichang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Jun,Jiang, Mengwei,Xiong, Jin,et al. Exploring a phage-based real-time pcr assay for diagnosing acinetobacter baumannii bloodstream infections with high sensitivity[J]. Analytica chimica acta,2018,1044:147-153. |
| APA | Luo, Jun.,Jiang, Mengwei.,Xiong, Jin.,Li, Junhua.,Zhang, Xiaoxu.,...&Yu, Junping.(2018).Exploring a phage-based real-time pcr assay for diagnosing acinetobacter baumannii bloodstream infections with high sensitivity.Analytica chimica acta,1044,147-153. |
| MLA | Luo, Jun,et al."Exploring a phage-based real-time pcr assay for diagnosing acinetobacter baumannii bloodstream infections with high sensitivity".Analytica chimica acta 1044(2018):147-153. |
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来源：武汉病毒研究所
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