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Evaluation of susceptibility of hiv-1 crf01_ae variants to neutralization by a panel of broadly neutralizing antibodies

文献类型：期刊论文

作者Wang, Hongye1,2; Yuan, Ting1; Li, Tingting1,2; Li, Yanpeng1; Qian, Feng3; Zhu, Chuanwu3; Liang, Shujia4; Hoffmann, Daniel5; Dittmer, Ulf6; Sun, Binlian1,7
刊名Archives of virology
出版日期2018-12-01
卷号163期号:12页码:3303-3315
ISSN号0304-8608
DOI10.1007/s00705-018-4011-7
通讯作者Sun, binlian(binlian17@jhun.edu.cn) ; Yang, rongge(ryang@wh.iov.cn)
英文摘要Broadly neutralizing antibodies (bnabs) are very promising agents for hiv-1 prophylaxis and aids treatment. however, the neutralization susceptibility of circulating recombinants such as crf01_ae, which is becoming increasingly prevalent, has not been studied in detail until now. here, we focused on crf01_ae in china and aimed to find bnabs that can be used for neutralization of crf01_ae. full-length env clones were obtained from the plasma samples of 22 hiv-1-infected individuals sampled in 2009 and 2015. an env-pseudovirus-based neutralization assay was conducted using five categories of bnabs: vrc01, nih45-46(g54w), and 3bnc117 (targeting the cd4 binding site); pg9 and pg16 (targeting the v1v2 loop); 2g12 (glycan specific), pgt121 and 10-1074 (targeting the v3 glycan); 2f5, 4e10, and 10e8 (targeting the membrane-proximal external region (mper)). the neutralizing efficiency was compared, and features of the escape pseudoviruses were analyzed. the crf01_ae pseudoviruses exhibited different susceptibility to these bnabs. overall, 4e10, 10e8, and 3bnc117 neutralized all 22 env-pseudotyped viruses, followed by nih45-46(g54w) and vrc01, which neutralized more than 90% of the viruses. 2f5, pg9, and pg16 showed only moderate breadth, while the other three bnabs neutralized none of these pseudoviruses. specifically, 10e8, nih45-46(g54w)and 3bnc117 showed the highest efficiency, combining neutralization potency and breadth. mutations at position 160, 169, 171 were associated with resistance to pg9 and pg16, while loss of a potential glycan at position 332 conferred insensitivity to v3-glycan-targeting bnabs. our results may help for choosing bnabs that can be used preferentially for prophylactic or therapeutic approaches in china.
WOS关键词IMMUNODEFICIENCY-VIRUS TYPE-1 ; HUMAN MONOCLONAL-ANTIBODY ; TREATMENT INTERRUPTION ; SEXUAL TRANSMISSION ; SUPPRESSES VIREMIA ; RATIONAL DESIGN ; ENV CLONES ; POTENT ; SUBTYPE ; CHINA
WOS研究方向Virology
WOS类目Virology
语种英语
WOS记录号WOS:000449845300009
出版者SPRINGER WIEN
URI标识http://www.irgrid.ac.cn/handle/1471x/2373225
专题武汉病毒研究所
通讯作者Sun, Binlian; Yang, Rongge
作者单位1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Res Grp HIV Mol Epidemiol & Virol 1, Wuhan 430071, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Fifth Peoples Hosp Suzhou, Div HIV AIDS 1, Suzhou 215000, Peoples R China
4.Guangxi Ctr Dis Control & Prevent, Dept HIV AIDS Control & Prevent, Nanning 530023, Peoples R China
5.Univ Duisburg Essen, Ctr Computat Sci & Simulat, Fac Biol, Ctr Med Biotechnol, D-45122 Essen, Germany
6.Univ Duisburg Essen, Univ Hosp Essen, Inst Virol, D-45122 Essen, Germany
7.Jianghan Univ, Sch Med, Dept Immunol, Wuhan 430000, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Hongye,Yuan, Ting,Li, Tingting,et al. Evaluation of susceptibility of hiv-1 crf01_ae variants to neutralization by a panel of broadly neutralizing antibodies[J]. Archives of virology,2018,163(12):3303-3315.
APA Wang, Hongye.,Yuan, Ting.,Li, Tingting.,Li, Yanpeng.,Qian, Feng.,...&Yang, Rongge.(2018).Evaluation of susceptibility of hiv-1 crf01_ae variants to neutralization by a panel of broadly neutralizing antibodies.Archives of virology,163(12),3303-3315.
MLA Wang, Hongye,et al."Evaluation of susceptibility of hiv-1 crf01_ae variants to neutralization by a panel of broadly neutralizing antibodies".Archives of virology 163.12(2018):3303-3315.

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