Screening and expressing hiv-1 specific antibody fragments in saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Wang, Ying1; Shan, Yaming4; Gao, Xinyu5,6; Gong, Rui5; Zheng, Jun2,3; Zhang, Xiaohua Douglas2,3; Zhao, Qi2,3 |
| 刊名 | Molecular immunology
 |
| 出版日期 | 2018-11-01 |
| 卷号 | 103页码:279-285 |
| 关键词 | Yeast display Saccharomyces cerevisiae Antibody expression Hiv-1 |
| ISSN号 | 0161-5890 |
| DOI | 10.1016/j.molimm.2018.10.013 |
| 通讯作者 | Zhao, qi(zhaoqi@alumni.cuhk.net) |
| 英文摘要 | Yeast displaying techniques have been widely used for identifying novel single-chain variable fragments (scfvs) and engineering their binding properties. in this study, we establish a set of vectors for scfv screening and production in the yeast system of saccharomyces cerevisiae. this suite includes a display vector pys for screening of recombinant scfv libraries as well as an expression vector pye for production of scfv candidates in saccharomyces cerevisiae. the display vector, pys, give the identification of the hiv-1-specific scfv clones from one scfv display library by fluorescence-activated cell sorting. subsequently, the expression vector pye can offer high quality scfvs of interest up to hundreds of microgram scale for bioactivity analysis. as the result, one identified scfv was confirmed to exhibit hiv-1 neutralization activity in a cell line-based pseudovirus assay. the advantage of this system enables the identical post-translation of mammalian scfvs in the same host cells. therefore, this vector set can be useful for the rapid screening and expression of antibody genes. |
| WOS关键词 | 2F5-LIKE ANTIBODIES ; PROTEIN-PRODUCTION ; PICHIA-PASTORIS ; YEAST DISPLAY ; BINDING ; AFFINITY ; PHAGE ; PURIFICATION ; RECEPTOR ; LIBRARY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Immunology |
| WOS类目 | Biochemistry & Molecular Biology ; Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000450382400031 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373226 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhao, Qi |
| 作者单位 | 1.Univ Macau, Inst Chinese Med Sci, Macau, Peoples R China 2.Univ Macau, Fac Hlth Sci, Canc Ctr, Macau, Peoples R China 3.Univ Macau, Fac Hlth Sci, Inst Translat Med, Macau, Peoples R China 4.Jilin Univ, Sch Life Sci, Natl Engn Lab AIDS Vaccine, Changchun, Jilin, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ying,Shan, Yaming,Gao, Xinyu,et al. Screening and expressing hiv-1 specific antibody fragments in saccharomyces cerevisiae[J]. Molecular immunology,2018,103:279-285. |
| APA | Wang, Ying.,Shan, Yaming.,Gao, Xinyu.,Gong, Rui.,Zheng, Jun.,...&Zhao, Qi.(2018).Screening and expressing hiv-1 specific antibody fragments in saccharomyces cerevisiae.Molecular immunology,103,279-285. |
| MLA | Wang, Ying,et al."Screening and expressing hiv-1 specific antibody fragments in saccharomyces cerevisiae".Molecular immunology 103(2018):279-285. |
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来源：武汉病毒研究所
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