Amino acid substitutions q129n and t131n/m133t in hepatitis b surface antigen (hbsag) interfere with the immunogenicity of the corresponding hbsag or viral replication ability
文献类型：期刊论文
| 作者 | Kang, Yaoyue1,3; Li, Fahong1; Guo, Hongying4; Yang, Sisi1; Zhang, Yongmei1; Zhu, Haoxiang1; Wang, Jinyu1; Mao, Richeng1; Qin, Yanli1; Xu, Jie3 |
| 刊名 | Virus research
 |
| 出版日期 | 2018-09-15 |
| 卷号 | 257页码:33-39 |
| ISSN号 | 0168-1702 |
| 关键词 | Hbsag Q129n T131n/m133t Antigenicity Immunogenicity Hbv replication |
| DOI | 10.1016/j.virusres.2018.08.019 |
| 通讯作者 | Wu, chunchen(wucc@wh.iov.cn) ; Zhang, jiming(jmzhang@fudan.edu.cn) |
| 英文摘要 | Variants of hepatitis b surface antigen (hbsag) influenced its antigenicity and immunogenicity. in our study, we aim to investigate biological significance of amino acid (aa) substitutions in hbsag, q129 n and t131 n/m133 t, for glycosylation, antigenicity and immunogenicity of variant hbsag (vthbsag) and viral replication. expression plasmids of vthbsag with aa substitutions q129 l, t123 n, q129 n and t131 n/m133 t were constructed. immunofluorescence (if) staining and western blot were simultaneously utilized to examine expression of vthbsag proteins in huh7 cells transfected with vthbsag constructs. vthbsag of q129 n and t131 n/m133 t created new n-glycosylation and displayed perinuclear distribution by if staining with the anti-ha. antigenicity of vthbsag of q129 n and t131 n/m133 t was reduced compared with wild type (wt) hbsag. in addition, we discovered impaired ability to induce anti-hbs responses against wthbsag in mice immunized with plasmids phbsag- q129 n and t131 n/m133 t. even so, efficient protective response toward wild type hbv can be primed by the two vthbsags in mice. further, we discovered that vthbsag with q129 n distinctly impaired hbv replication capacity, but vthbsag with t131 n/m133 t had no impact on viral replication. thus, we conclude that vthbsag with q129 n or t131 n/m133 t creates new n-glycosylation and interferes with both the antigenicity and immunogenicity of vthbsag. and vthbsag with q129 n impaired hbv replication ability. |
| WOS关键词 | CLINICAL-SIGNIFICANCE ; VACCINATION PROGRAM ; GLYCOSYLATION SITE ; VIRUS REPLICATION ; VIRION SECRETION ; IMMUNE ESCAPE ; BLOOD-DONORS ; MUTATIONS ; INFECTION ; PROTEIN |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000448090500005 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373232 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wu, Chunchen; Zhang, Jiming |
| 作者单位 | 1.Fudan Univ, Huashan Hosp, Shanghai Med Coll, Dept Infect Dis, Shanghai 200040, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 3.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Infect Dis, Sch Med, Shanghai, Peoples R China 4.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Dept Hepatitis Dis, Shanghai, Peoples R China 5.Fudan Univ, Minist Educ, Key Lab Med Mol Virol, Shanghai, Peoples R China 6.Fudan Univ, Minist Hlth, Key Lab Med Mol Virol, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kang, Yaoyue,Li, Fahong,Guo, Hongying,et al. Amino acid substitutions q129n and t131n/m133t in hepatitis b surface antigen (hbsag) interfere with the immunogenicity of the corresponding hbsag or viral replication ability[J]. Virus research,2018,257:33-39. |
| APA | Kang, Yaoyue.,Li, Fahong.,Guo, Hongying.,Yang, Sisi.,Zhang, Yongmei.,...&Zhang, Jiming.(2018).Amino acid substitutions q129n and t131n/m133t in hepatitis b surface antigen (hbsag) interfere with the immunogenicity of the corresponding hbsag or viral replication ability.Virus research,257,33-39. |
| MLA | Kang, Yaoyue,et al."Amino acid substitutions q129n and t131n/m133t in hepatitis b surface antigen (hbsag) interfere with the immunogenicity of the corresponding hbsag or viral replication ability".Virus research 257(2018):33-39. |
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来源：武汉病毒研究所
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