Establishment of baculovirus-expressed vlps induced syncytial formation assay for flavivirus antiviral screening
文献类型：期刊论文
| 作者 | Dai, Shiyu1,2; Zhang, Yanfang1; Zhang, Tao1; Zhang, Bo1; Wang, Hualin1; Deng, Fei1 |
| 刊名 | Viruses-basel
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 10期号:7页码:19 |
| ISSN号 | 1999-4915 |
| 关键词 | Baculovirus expression system Virus like particle Flavivirus Zika virus Syncytia |
| DOI | 10.3390/v10070365 |
| 通讯作者 | Wang, hualin(h.wang@wh.iov.cn) ; Deng, fei(df@wh.iov.cn) |
| 英文摘要 | The baculovirus-insect cell expression system has been widely used for heterologous protein expression and virus-like particles (vlps) expression. in this study, we established a new method for antiviral screening targeting to glycoprotein e of flaviviruses based on the baculovirus expression system. zikv is a mosquito-borne flavivirus and has posed great threat to the public health. it has been reported that zikv infection was associated with microcephaly and serious neurological complications. our study showed that either zikv e or prme protein expressed in insect cells can form vlps and induce membrane fusion between insect cells. therefore, the e protein, which is responsible for receptor binding, attachment, and virus fusion during viral entry, achieved proper folding and retained its fusogenic ability in vlps when expressed in this system. the syncytia in insect cells were significantly reduced by the anti-zikv-e specific polyclonal antibody in a dose-dependent manner. ams, a thiol-conjugating reagent, was also shown to have an inhibitory effect on the e protein induced syncytia and inhibited zikv infection by blocking viral entry. indeed the phenomenon of syncytial formation induced by e protein expressed vlps in insect cells is common among flaviviruses, including japanese encephalitis virus (jev), dengue virus type 2 (denv-2), and tick-borne encephalitis virus (tbev). this inhibition effect on syncytial formation can be developed as a novel, safe, and simple antiviral screening approach for inhibitory antibodies, peptides, or small molecules targeting to e protein of zikv and other flaviviruses. |
| WOS关键词 | VIRUS-LIKE PARTICLES ; INSECT-CELL LINE ; ZIKA VIRUS ; PERSISTENT INFECTION ; CYSTEINE RESIDUES ; ENVELOPE PROTEIN ; SF9 CELLS ; VACCINE ; FUSION ; ENTRY |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000445153200027 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373242 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Hualin; Deng, Fei |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Shiyu,Zhang, Yanfang,Zhang, Tao,et al. Establishment of baculovirus-expressed vlps induced syncytial formation assay for flavivirus antiviral screening[J]. Viruses-basel,2018,10(7):19. |
| APA | Dai, Shiyu,Zhang, Yanfang,Zhang, Tao,Zhang, Bo,Wang, Hualin,&Deng, Fei.(2018).Establishment of baculovirus-expressed vlps induced syncytial formation assay for flavivirus antiviral screening.Viruses-basel,10(7),19. |
| MLA | Dai, Shiyu,et al."Establishment of baculovirus-expressed vlps induced syncytial formation assay for flavivirus antiviral screening".Viruses-basel 10.7(2018):19. |
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来源：武汉病毒研究所
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