Tick-borne encephalitis virus nonstructural protein ns5 induces rantes expression dependent on the rna-dependent rna polymerase activity
文献类型：期刊论文
| 作者 | Zheng, Zifeng1,2; Yang, Jieyu2,3; Jiang, Xuan1; Liu, Yalan1; Zhang, Xiaowei1; Li, Mei1,2; Zhang, Mudan1,4; Fu, Ming1,2; Hu, Kai1; Wang, Hanzhong3 |
| 刊名 | Journal of immunology
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 201期号:1页码:53-68 |
| ISSN号 | 0022-1767 |
| DOI | 10.4049/jimmunol.1701507 |
| 通讯作者 | Liu, yalan(liuyl@wh.iov.cn) ; Hu, qinxue(qhu@wh.iov.cn) |
| 英文摘要 | Tick-borne encephalitis virus (tbev) is one of the flaviviruses that targets the cns and causes encephalitis in humans. the mechanism of tbev that causes cns destruction remains unclear. it has been reported that rantes-mediated migration of human blood monocytes and t lymphocytes is specifically induced in the brain of mice infected with tbev, which causes ensuing neuroinflammation and may contribute to brain destruction. however, the viral components responsible for rantes induction and the underlying mechanisms remain to be fully addressed. in this study, we demonstrate that the ns5, but not other viral proteins of tbev, induces rantes production in human glioblastoma cell lines and primary astrocytes. tbev ns5 appears to activate the ifn regulatory factor 3 (irf-3) signaling pathway in a manner dependent on rig-i/mda5, which leads to the nuclear translocation of irf-3 to bind with rantes promoter. further studies reveal that the activity of rna-dependent rna polymerase (rdrp) but not the rna cap methyltransferase is critical for tbev ns5-induced rantes expression, and this is likely due to rdrp-mediated synthesis of dsrna. additional data indicate that the residues at k359, d361, and d664 of tbev ns5 are critical for rdrp activity and rantes induction. of note, ns5s from other flaviviruses, including japanese encephalitis virus, west nile virus, zika virus, and dengue virus, can also induce rantes expression, suggesting the significance of ns5-induced rantes expression in flavivirus pathogenesis. our findings provide a foundation for further understanding how flaviviruses cause neuroinflammation and a potential viral target for intervention. |
| WOS关键词 | INTERFERON REGULATORY FACTOR-3 ; DOUBLE-STRANDED-RNA ; HUMAN CYTOMEGALOVIRUS-INFECTION ; NEURAL PROGENITOR CELLS ; NF-KAPPA-B ; EPITHELIAL-CELLS ; IMMUNE-RESPONSE ; WEST-NILE ; TRANSCRIPTION FACTORS ; INFLAMED ENDOTHELIUM |
| WOS研究方向 | Immunology |
| WOS类目 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000442387100008 |
| 出版者 | AMER ASSOC IMMUNOLOGISTS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373246 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Liu, Yalan; Hu, Qinxue |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Ctr Emerging Infect Dis, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 4.Guangzhou Women & Childrens Med Ctr, Guangzhou Inst Pediat, Guangzhou 510623, Guangdong, Peoples R China 5.St Georges Univ London, Inst Infect & Immun, London SW17 0RE, England |
| 推荐引用方式 GB/T 7714 | Zheng, Zifeng,Yang, Jieyu,Jiang, Xuan,et al. Tick-borne encephalitis virus nonstructural protein ns5 induces rantes expression dependent on the rna-dependent rna polymerase activity[J]. Journal of immunology,2018,201(1):53-68. |
| APA | Zheng, Zifeng.,Yang, Jieyu.,Jiang, Xuan.,Liu, Yalan.,Zhang, Xiaowei.,...&Hu, Qinxue.(2018).Tick-borne encephalitis virus nonstructural protein ns5 induces rantes expression dependent on the rna-dependent rna polymerase activity.Journal of immunology,201(1),53-68. |
| MLA | Zheng, Zifeng,et al."Tick-borne encephalitis virus nonstructural protein ns5 induces rantes expression dependent on the rna-dependent rna polymerase activity".Journal of immunology 201.1(2018):53-68. |
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来源：武汉病毒研究所
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