Characteristics and optimised fermentation of a novel magnetotactic bacterium, magnetospirillum sp me-1
文献类型：期刊论文
| 作者 | Ke, Linfeng1,2; Chen, Yajun1,2; Liu, Pengming1; Liu, Shan1,2; Wu, Dandan1; Yuan, Yihui1; Wu, Yan1; Gao, Meiying1 |
| 刊名 | Fems microbiology letters
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 365期号:14页码:9 |
| 关键词 | Magnetospirillum sp me-1 Isolation Genomic analysis Optimised fermentation Fed-batch cultivation |
| ISSN号 | 0378-1097 |
| DOI | 10.1093/femsle/fny052 |
| 通讯作者 | Gao, meiying(mygao@wh.iov.cn) |
| 英文摘要 | Magnetotactic bacteria (mtb) can biosynthesise magnetosomes, which have great potential for commercial applications. a new mth strain, magnetospirillum sp. me-1, was isolated and cultivated from freshwater sediments of east lake (wuhan, china) using the limiting dilution method. me-1 had a chain of 17 +/- 4 magnetosomes in the form of cubooctahedral crystals with a shape factor of 0.89. me-1 was closest to magnetospirillum sp. xm-1 according to 16s rrna gene sequence similarity. compared with xm-1, me-1 possessed an additional copy of mampa and a larger mamo in magnetosome-specific genes. me-1 had an intact citric acid cycle, and complete pathway models of ammonium assimilation and dissimilatory nitrate reduction. potential carbon and nitrogen sources in these pathways were confirmed to be used in me-1. adipate was determined to be used in the fermentation medium as a new kind of dicarboxylic acid. the optimised fermentation medium was determined by orthogonal tests. the large-scale production of magnetosomes was achieved and the magnetosome yield (wet weight) reached 120 mg l-1 by fed-batch cultivation of me-1 at 49 h in a 10-l fermenter with the optimised fermentation medium. this study may provide insights into the isolation and cultivation of other new mtb strains and the production of magnetosomes. |
| WOS关键词 | OXYGEN-CONTROLLED FERMENTER ; FED-BATCH CULTURE ; MAGNETOSOME FORMATION ; AQUASPIRILLUM-MAGNETOTACTICUM ; GRYPHISWALDENSE MSR-1 ; MAGNETIC SPIRILLUM ; BETA-OXIDATION ; SP-NOV ; GROWTH ; AMB-1 |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000441114900012 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373250 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gao, Meiying |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ke, Linfeng,Chen, Yajun,Liu, Pengming,et al. Characteristics and optimised fermentation of a novel magnetotactic bacterium, magnetospirillum sp me-1[J]. Fems microbiology letters,2018,365(14):9. |
| APA | Ke, Linfeng.,Chen, Yajun.,Liu, Pengming.,Liu, Shan.,Wu, Dandan.,...&Gao, Meiying.(2018).Characteristics and optimised fermentation of a novel magnetotactic bacterium, magnetospirillum sp me-1.Fems microbiology letters,365(14),9. |
| MLA | Ke, Linfeng,et al."Characteristics and optimised fermentation of a novel magnetotactic bacterium, magnetospirillum sp me-1".Fems microbiology letters 365.14(2018):9. |
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来源：武汉病毒研究所
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