The transmembrane nucleoporin pom121 ensures efficient hiv-1 pre-integration complex nuclear import
文献类型：期刊论文
| 作者 | Guo, Jing1,2; Liu, Xianxian1,2; Wu, Chuanjian1,2; Hu, Jingping1,2; Peng, Ke3; Wu, Li4; Xiong, Sidong1,2; Dong, Chunsheng1,2 |
| 刊名 | Virology
 |
| 出版日期 | 2018-08-01 |
| 卷号 | 521页码:169-174 |
| 关键词 | Hiv-1 Pom121 Nuclear import Pre-integration complex Nucleoporins |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2018.06.008 |
| 通讯作者 | Xiong, sidong(sdxiong@suda.edu.cn) ; Dong, chunsheng(chunshengdong@suda.edu.cn) |
| 英文摘要 | Hiv-1 hijacks host classical cargo nuclear transportation, or nonclassical pathways by directly interacting with importin-beta family proteins or nucleoporins for efficient pre-integration complex (pic) nuclear import. recently, an n-terminal truncated form of nucleoporin pom121c (601-987 aa) was reported to inhibit hiv-1 replication. in contrast, we found that hiv-1 replication was significantly decreased in 293t and tzm-bl cells with sirna-mediated pom121 knockdown. quantitative pcr indicated that viral replication was impaired at the step of cdna nuclear import. furthermore, we found that karyopherin-beta 1 (kpnb1), which belongs to the importin-beta family, interacts with pom121 and is involved in pom121-mediated pic nuclear import. rescue experiment indicated that the fg-repeats and the following alpha-helix in pom121 are required for its role in hiv-1 pic nuclear import. taken together, our results showed that full-length pom121 enables efficient pic nuclear import, and suggested that this process may rely on kpnb1 dependent classical cargo nuclear transportation way. |
| WOS关键词 | IMMUNODEFICIENCY-VIRUS TYPE-1 ; PORE COMPLEX ; NUCLEOCYTOPLASMIC TRANSPORT ; MEMBRANE ; REPLICATION ; CELLS ; ENTRY ; INTERPHASE ; INTEGRASE ; INTERACTS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000439956400018 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373252 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xiong, Sidong; Dong, Chunsheng |
| 作者单位 | 1.Soochow Univ, Inst Biol, Jiangsu Key Lab Infect & Immun, Suzhou 215123, Peoples R China 2.Soochow Univ, Inst Med Sci, Jiangsu Key Lab Infect & Immun, Suzhou 215123, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 4.Ohio State Univ, Dept Vet Biosci, Ctr Retrovirus Res, Columbus, OH 43210 USA |
| 推荐引用方式 GB/T 7714 | Guo, Jing,Liu, Xianxian,Wu, Chuanjian,et al. The transmembrane nucleoporin pom121 ensures efficient hiv-1 pre-integration complex nuclear import[J]. Virology,2018,521:169-174. |
| APA | Guo, Jing.,Liu, Xianxian.,Wu, Chuanjian.,Hu, Jingping.,Peng, Ke.,...&Dong, Chunsheng.(2018).The transmembrane nucleoporin pom121 ensures efficient hiv-1 pre-integration complex nuclear import.Virology,521,169-174. |
| MLA | Guo, Jing,et al."The transmembrane nucleoporin pom121 ensures efficient hiv-1 pre-integration complex nuclear import".Virology 521(2018):169-174. |
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来源：武汉病毒研究所
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